dhs-16 Antibody
Description
Introduction to DHS-16 Antibody
The term "DHS-16 Antibody" likely refers to an antibody targeting Deoxyhypusine Synthase (DHPS), a critical enzyme in the post-translational modification of eukaryotic translation initiation factor 5A (eIF5A). DHPS catalyzes the formation of deoxyhypusine, a precursor to hypusine, which is essential for eIF5A’s role in cell proliferation and apoptosis regulation. While "DHS-16" is not explicitly defined in public literature, the closest validated antibody is Anti-DHS DHPS Antibody (A02106) from Boster Bio, which targets human DHPS and is widely used in research applications .
Target Protein: DHPS
Key Features of DHPS:
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Molecular Weight: ~39 kDa (observed), 40,971 Da (calculated) .
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Function: Catalyzes the NAD-dependent activation of the ε-amino group of a lysine residue in eIF5A to form deoxyhypusine.
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Pathway Involvement: Critical for cellular processes like mRNA translation, inflammation, and cancer progression.
Table 1: Antibody Characteristics
| Parameter | Details |
|---|---|
| Host Species | Rabbit (Polyclonal IgG) |
| Immunogen | Synthesized peptide (residues 51–100 of human DHPS) |
| Reactivity | Human |
| Applications | Western Blot (WB) |
| Dilution Range | 1:500 – 1:2000 |
| Storage | -20°C (long-term); 4°C (short-term) |
Western Blot (WB) Performance
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Validation: The antibody detects DHPS in lysates from human cell lines (e.g., K562 leukemic cells) .
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Example Data:
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Band observed at ~39 kDa in WB, consistent with DHPS’s molecular weight.
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Specificity confirmed using blocking peptides and negative controls.
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Table 2: Key Validation Metrics
| Metric | Result |
|---|---|
| Observed MW | 39 kDa |
| Signal-to-Noise | High (clear band resolution) |
| Cross-Reactivity | None reported (human-specific) |
Biological Relevance of DHPS
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Cancer Research: DHPS overexpression is linked to tumor growth and metastasis. Antibodies like A02106 enable studies on DHPS’s role in oncogenesis .
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Infectious Disease: Hypusinated eIF5A is required for HIV-1 replication, making DHPS a potential therapeutic target.
Limitations and Future Directions
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- DHS-16 regulates DAF-12 activity, which in turn governs longevity in response to signals originating from the gonad. PMID: 22505847
