DLL4 Human
Description
DLL4 Human Recombinant produced in HEK293 is a single glycosylated polypeptide chain containing 504 amino acids (27-529 a.a) and having a molecular mass of 55.1kDa. DLL4 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Delta-Like4 (DLL4) is a Notch ligand involved in Notch signaling. It acts as a negative regulator of endothelial cell proliferation, migration, and angiogenic sprouting. DLL4 plays a critical role in retinal progenitor proliferation and is essential for suppressing rod cell fates in late retinal progenitors. It is also necessary for the proper development of other retinal cell types. In spinal cord neurogenesis, DLL4 inhibits the differentiation of V2a interneurons at specific developmental stages.
Recombinant human DLL4 protein, expressed in HEK293 cells, is a single glycosylated polypeptide chain consisting of 504 amino acids (residues 27-529). It has a molecular weight of 55.1 kDa. The DLL4 protein includes a C-terminal 6-amino acid His-tag and is purified using proprietary chromatographic methods.
The DLL4 protein solution has a concentration of 0.25 mg/ml and is formulated in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
The purity is determined to be greater than 95.0% by SDS-PAGE analysis.
Delta-like protein 4, Drosophila Delta homolog 4, Delta4, delta like canonical Notch ligand 4, AOS6, hdelta2.
HEK293.
SGVFQLQLQE FINERGVLAS GRPCEPGCRT FFRVCLKHFQ AVVSPGPCTF GTVSTPVLGT NSFAVRDDSS GGGRNPLQLP FNFTWPGTFS LIIEAWHAPG DDLRPEALPP DALISKIAIQ GSLAVGQNWL LDEQTSTLTR LRYSYRVICS DNYYGDNCSR LCKKRNDHFG HYVCQPDGNL SCLPGWTGEY CQQPICLSGC HEQNGYCSKP AECLCRPGWQ GRLCNECIPH NGCRHGTCST PWQCTCDEGW GGLFCDQDLN YCTHHSPCKN GATCSNSGQR SYTCTCRPGY TGVDCELELS ECDSNPCRNG GSCKDQEDGY HCLCPPGYYG LHCEHSTLSC ADSPCFNGGS CRERNQGANY ACECPPNFTG SNCEKKVDRC TSNPCANGGQ CLNRGPSRMC RCRPGFTGTY CELHVSDCAR NPCAHGGTCH DLENGLMCTC PAGFSGRRCE VRTSIDACAS SPCFNRATCY TDLSTDTFVC NCPYGFVGSR CEFPVGLPHH HHHH.
Product Science Overview
Delta-Like 4 (DLL4) is a protein that plays a crucial role in the Notch signaling pathway, which is essential for various cellular processes, including differentiation, proliferation, and apoptosis. DLL4 is a homolog of the Drosophila delta protein and functions as a transmembrane ligand for the Notch receptors, primarily Notch1 and Notch4 .
DLL4 is characterized by a DSL (Delta/Serrate/Lag-2) domain, multiple EGF (epidermal growth factor) repeats, and a transmembrane domain . It is predominantly expressed in the vascular endothelium and is induced by vascular endothelial growth factor (VEGF) . The expression of DLL4 is tightly regulated and is critical for proper vascular development and angiogenesis .
DLL4 is a key regulator of angiogenesis, the process by which new blood vessels form from pre-existing vessels . It acts as a negative feedback regulator in response to VEGF, controlling angiogenic sprouting and promoting the formation of a differentiated vascular network . DLL4’s role in angiogenesis is crucial for maintaining the balance between vascular proliferation and maturation .
DLL4 has been found to be strongly expressed in tumor vessels, particularly in clear-cell renal tumors and bladder cancer . Inhibition of DLL4 results in increased vascular proliferation but defective maturation, leading to a decrease in tumor growth without apparent toxicity . This makes DLL4 a potential target for anti-cancer therapies aimed at disrupting tumor angiogenesis.
Human recombinant DLL4 proteins are produced using various expression systems, such as HEK293 cells . These recombinant proteins are used in a range of applications, including ELISA, functional assays, Western blotting, and immunohistochemistry . They are valuable tools for studying the Notch signaling pathway and its role in vascular development and disease.
