DMRT3 Antibody, HRP conjugated
Description
Role in Cancer and Immunotherapy
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Prognostic Marker: Elevated DMRT3 expression correlates with poor survival in kidney renal clear cell carcinoma (KIRC), lung adenocarcinoma (LUAD), and uterine corpus endometrial carcinoma (UCEC) .
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Immune Modulation: DMRT3 interacts with tumor microenvironment (TME) components, influencing immune checkpoint genes (e.g., PD-1, CTLA-4) and immune cell infiltration .
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Chemoresistance: High DMRT3 levels associate with resistance to cisplatin and paclitaxel, suggesting utility in predicting therapeutic outcomes .
Neurological and Developmental Studies
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Spinal Circuit Development: DMRT3 is essential for configuring spinal interneurons that coordinate limb movement, validated in murine models .
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Sexual Differentiation: Regulates transcriptional events during gonadal development, particularly in testis-specific pathways .
Enhanced Detection via HRP Conjugation
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Lyophilization Efficiency: A modified conjugation protocol involving HRP lyophilization post-oxidation increases antibody-enzyme binding capacity, improving ELISA sensitivity (1:5000 dilution vs. 1:25 in classical methods) .
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Stability: HRP conjugates retain activity for >12 months at -20°C when stored in glycerol-based buffers .
Protocol Optimization
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ELISA: Use blocking buffers (e.g., 5% BSA) to reduce non-specific binding. Optimal antigen-antibody incubation: 1 hour at 37°C .
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Western Blot: Resolve proteins on 8–12% SDS-PAGE gels. Transfer to PVDF membranes and detect using chemiluminescent substrates (e.g., ECL) .
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IHC: Antigen retrieval (e.g., citrate buffer, pH 6.0) enhances signal in paraffin-embedded tissues .
Validation Metrics
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Specificity: Confirm via knockout cell lines or competitive assays with fusion proteins .
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Sensitivity: Detect ≤10 ng of recombinant DMRT3 in WB under optimized conditions .
Future Directions
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- A study on the genetic basis of cerebral palsy (CP) found that the deletion of the cis-regulatory element for DMRT3 in humans may cause impaired development of the forebrain and gait abnormalities, resulting in spastic CP. This study provides new insights into the genetic basis of CP. PMID: 29305858
- Chromosome 9p loss is a characteristic feature of squamous cell carcinoma. The DMRT1, DMRT3, and DOCK8 genes located at 9p24.3 are potentially interesting targets for therapeutic measures in the study of the pathophysiology of squamous cell carcinoma. PMID: 20596660
