ATJ15 Antibody
Description
Overview of ADAMTS15 Antibody
ADAMTS15 (A Disintegrin and Metalloproteinase with Thrombospondin Motifs 15) is a secreted protease involved in extracellular matrix remodeling and tumor suppression . Antibodies targeting ADAMTS15, such as MAB5149 (R&D Systems), are widely used in research to study its expression, localization, and functional roles .
Functional Roles and Clinical Relevance
ADAMTS15 exhibits context-dependent roles in cancer biology:
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Tumor Suppression: Downregulated in colon cancer and inversely correlated with metastasis .
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Prognostic Marker: High expression in breast cancer associates with poor survival outcomes .
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Therapeutic Target: Potential utility in antibody-drug conjugates (ADCs) for targeted cancer therapy, as demonstrated by analogous anti-Tn antibody-MMAF conjugates .
Research Applications and Validation Data
Table 2: Diagnostic Sensitivity and Specificity in ELISA
| Sample Type | Sensitivity (95% CI) | Specificity (95% CI) | Reference |
|---|---|---|---|
| Serum | 0.45 (0.29–0.63) | 0.93 (0.86–0.97) | |
| Plasma | 0.38 (0.22–0.56) | 0.89 (0.81–0.94) |
Mechanistic Insights from Preclinical Studies
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Internalization Efficiency: Anti-Tn antibodies like Chi-Tn mAb demonstrate rapid internalization (~30 minutes), enabling targeted drug delivery .
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Cytotoxicity: Conjugation with auristatin F (MMAF) yields IC₅₀ values as low as 7 × 10⁻¹¹ M in Jurkat cells .
Limitations and Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- A significant number of genes exhibiting altered expression in spaceflight conditions within wild-type cells were found to be dependent on Arg1, indicating a major role for this gene in the physiological adaptation of undifferentiated cells to spaceflight. PMID: 29088549
- Under ammonium stress, the Arabidopsis mutant gsa-1 (gravitropism sensitive to ammonium-1) displayed increased loss of root gravitropism. Notably, disruption of GSA-1/ARG1 can reduce basipetal auxin transport and the expression of AUX1 protein in root apices. [ARG1] [ALTERED RESPONSE TO GRAVITY1] PMID: 23782229
