Customize dpf-6 Antibody

Description

Potential Nomenclature Considerations

DPF-3 Antibody (mentioned in Source ):

Target: Dipeptidyl peptidase DPF-3 (C. elegans ortholog of mammalian DPP8/9)
Function: Regulates microRNA Argonaute proteins (ALG-1/ALG-2) by controlling mRNA processing and protein stability .
Research Findings:
- DPF-3 interacts with ALG-1 to gatekeep compensatory mechanisms in miRNA pathways .
- Knockout of dpf-3 restores miRNA function in alg-1 mutants, improving organismal fitness .

DEF6 Antibody (Source ):

Target: Differentially Expressed in FDCP 6 (DEF6), a guanine nucleotide exchange factor.
Function: Regulates Rho GTPases (RAC1, CDC42) and immune homeostasis via CTLA-4 trafficking .
Validation:
- Western blot: Detects DEF6 at ~74 kDa in Jurkat cell lysates .
- Flow cytometry: Validated in permeabilized human cells .

Comparative Analysis of Related Antibodies

Antibody	Target Protein	Species Reactivity	Key Applications
DPF-3	Dipeptidyl peptidase	C. elegans	miRNA pathway regulation
DEF6	Guanine nucleotide exchanger	Human	Immune regulation, GTPase signaling
PDIA6	Protein disulfide isomerase	Human, Rat	Chaperone activity, platelet aggregation

Research Gaps and Recommendations

Nomenclature Clarification: "dpf-6" is not documented in the provided sources. Potential candidates include DEF6 (immune regulator) or DPF-3 (peptidase in miRNA pathways).
Experimental Validation: For DEF6, functional studies show its role in T-cell regulation . For DPF-3, genetic interaction data highlight its role in Argonaute compensation .

Key Citations

DPF-3: Regulates miRNA Argonaute ALG-2 mRNA processing in C. elegans .
DEF6: Essential for immune homeostasis and Th2 cell activation .

Product Specs

Buffer

Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

14-16 Weeks (Made-to-Order)

Synonyms

dpf-6 antibody; F44B9.1Dipeptidyl peptidase family member 6 antibody; EC 3.4.14.- antibody

Target Names

dpf-6

Uniprot No.

P34422

Target Background

Function

This antibody targets a protein involved in the sequential removal of N-terminal dipeptides from polypeptides. This function is crucial for regulating distal tip cell migration.

Database Links

KEGG: cel:CELE_F44B9.1

STRING: 6239.F44B9.1a

UniGene: Cel.6344

Protein Families

Peptidase S9B family, DPPIV subfamily

Subcellular Location

Cell membrane; Single-pass type II membrane protein.

Q&A

Here’s a structured FAQ collection for researchers working with dpf-6 Antibody, designed to address academic research challenges while incorporating methodological guidance and evidence-based insights:

Advanced Research Questions

How can conflicting data from dpf-6 antibody-based assays (e.g., IHC vs. ELISA) be resolved?

Troubleshooting framework:
- Verify antigen integrity (e.g., pH-dependent epitope stability in IHC fixatives) .
- Compare antibody performance across buffer conditions (e.g., Tris vs. PBS).
- Use quantitative Western blotting with recombinant protein standards to calibrate signal linearity .

What strategies improve dpf-6 antibody performance in multiplex assays (e.g., spatial proteomics)?

Optimize epitope compatibility by:
- Validating antibody cross-reactivity using peptide arrays or in silico epitope mapping.
- Employing sequential staining with antibody stripping between rounds.
- Testing fluorophore combinations to minimize spectral overlap .

How should researchers address dpf-6 antibody cross-reactivity in phylogenetically related species?

Methodology:
- Perform phylogenetic footprinting to identify conserved vs. divergent epitope regions.
- Validate using tissues from species with annotated genome sequences.
- Generate chimeric proteins to isolate cross-reactive domains .

Data Interpretation & Reproducibility

What statistical approaches are recommended for analyzing dpf-6 antibody-derived proteomic datasets?

Apply:
- Benjamini-Hochberg correction for multiple comparisons in high-throughput screens.
- Intra-class correlation (ICC) to assess inter-experiment consistency.
- Tools like Perseus or Skyline for label-free quantification normalization .

How can researchers distinguish between technical artifacts and biological signals in dpf-6 antibody-based imaging?

Controls to implement:
- Isogenic KO controls (gold standard for specificity).
- Threshold-based segmentation to filter background noise.
- Independent validation via RNA-FISH or CRISPR-Cas9-mediated protein tagging .

Ethical & Reporting Standards

What metadata must accompany publications using dpf-6 antibody?

Include:
- RRID (Research Resource Identifier) for traceability.
- Batch-specific validation data (e.g., lot number, storage conditions).
- Detailed protocols via platforms like Protocols.io .

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dpf-6 Antibody