DRT100 Antibody
Description
DRT100 Antibody in Plant Biology
The DRT100 antibody (Product Code: CSB-PA184095XA01DOA) is a research tool designed for studying the DRT100 protein in Arabidopsis thaliana (Mouse-ear cress).
DRT100 ELISA Kit for Human sTNF-RI Quantification
The Quantikine® Human TNF RI/TNFRSF1A Immunoassay (Catalog #DRT100) is a 4.5-hour sandwich ELISA for measuring soluble TNF-RI in biological fluids.
Principle of the Assay:
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Capture Antibody: Monoclonal anti-human TNF-RI pre-coated on microplates.
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Detection Antibody: Polyclonal anti-human TNF-RI conjugated to horseradish peroxidase.
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Signal Detection: Tetramethylbenzidine (TMB) substrate, measured at 450 nm .
Performance Metrics:
| Parameter | Value |
|---|---|
| Sensitivity | 0.77 pg/mL (mean MDD) |
| Assay Range | 31.2–2000 pg/mL |
| Intra-Assay CV | 3.5–5.1% |
| Inter-Assay CV | 6.7–8.8% |
Linearity Data in Human Samples:
| Sample Type | 1:2 Dilution (% Recovery) | 1:16 Dilution (% Recovery) |
|---|---|---|
| Serum | 99–115% | 77–114% |
| EDTA Plasma | 93–107% | 85–106% |
| Urine | 97–114% | 81–118% |
A. Baseline sTNF-RI Levels in Healthy Donors
| Sample Type | Mean (pg/mL) | Range (pg/mL) |
|---|---|---|
| Serum (n=40) | 1198 | 749–1966 |
| EDTA Plasma (n=40) | 914 | 484–1407 |
| Urine (n=33) | 1029 | 173–4030 |
B. sTNF-RI in Stimulated Cell Cultures
Peripheral blood mononuclear cells treated with phytohemagglutinin (PHA) showed rising sTNF-RI levels:
| Stimulant | Day 1 (pg/mL) | Day 3 (pg/mL) | Day 5 (pg/mL) |
|---|---|---|---|
| PHA | 24 | 76 | 141 |
| LPS | 17 | 36 | 49 |
This kit is critical for studies on inflammatory diseases, as sTNF-RI modulates TNF-α activity and correlates with autoimmune conditions like rheumatoid arthritis .
Comparative Analysis of DRT100 Reagents
| Feature | DRT100 Antibody (Plant) | DRT100 ELISA Kit (Human) |
|---|---|---|
| Target | Arabidopsis DRT100 protein | Soluble TNF-RI in humans |
| Primary Use | Protein localization/expression | Biomarker quantification |
| Key Data | Uniprot Q00874 binding | Sensitivity: 0.77 pg/mL |
| Commercial Source | Cusabio | R&D Systems/Bio-Techne |
Technical Considerations
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Cross-Reactivity: The DRT100 ELISA shows ≤0.5% cross-reactivity with TNF-α, TNF-β, or other TNF receptors .
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Sample Stability: Serum/plasma samples remain stable for ≥6 months at -80°C .
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Clinical Relevance: Elevated sTNF-RI levels are observed in chronic inflammation and predict poor responses to anti-TNF therapies .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
