DREB1E Antibody

What is DREB1E?

DREB1E belongs to the DREB1/CBF (C-repeat binding factor) family of transcription factors, which regulate stress-responsive genes by binding to dehydration-responsive elements (DREs) in plant promoters . In rice (Oryza sativa), OsDREB1E is a homolog of Arabidopsis CBF genes and functions as a positive regulator of:

• Chilling tolerance via reactive oxygen species (ROS) scavenging and cell death regulation .

• Heat tolerance through transcriptional activation of heat-stress genes .

• Cross-stress adaptation, as overexpression enhances resistance to multiple abiotic stressors .

Research Findings on DREB1E Function

Studies in rice highlight DREB1E’s role in stress adaptation:

• Transcriptional Regulation: OsDREB1E activates genes involved in ROS scavenging (e.g., peroxidases) and inhibits cell death under chilling stress .

• Cross-Stress Tolerance: Overexpression of OsDREB1E improves heat, salt, and drought tolerance, suggesting broad regulatory networks .

• Divergence from Arabidopsis: Unlike Arabidopsis CBFs, rice DREB1E does not mediate cold acclimation but directly enhances basal stress tolerance .

Comparative Analysis of DREB1 Proteins

Challenges and Future Directions

• Antibody Specificity: Existing DREB1 antibodies (e.g., anti-DREB1B) may cross-react with DREB1E due to conserved AP2 DNA-binding domains. Validation via knockout mutants is critical .

• Functional Studies: Further research is needed to map DREB1E’s interaction partners and downstream targets using ChIP-seq .

• Biotechnological Potential: Engineering crops with DREB1E overexpression could enhance climate resilience, though trade-offs (e.g., growth retardation) require optimization .