At5g18400 Antibody
Description
Definition and Target Specificity
The At5g18400 antibody targets the protein encoded by the AT5G18400 gene in Arabidopsis thaliana. This gene is annotated as Cytokine-induced anti-apoptosis inhibitor 1 and is implicated in Fe-S cluster biogenesis, a critical process for mitochondrial function and enzyme activity . The antibody’s specificity is confirmed by its UniProt ID (Q8L7Z3) and standardized validation protocols .
Antigen Details
| Property | Detail |
|---|---|
| Gene Name | AT5G18400 |
| Protein Function | Anti-apoptosis regulation, Fe-S cluster assembly |
| UniProt ID | Q8L7Z3 |
| Species | Arabidopsis thaliana (Mouse-ear cress) |
Research Applications
The At5g18400 antibody is primarily utilized in:
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Western Blotting: Detecting protein expression levels under stress conditions.
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Immunoprecipitation: Isolating the AT5G18400 protein for interaction studies.
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Cellular Localization: Mapping subcellular distribution via immunofluorescence .
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Functional Studies: Investigating its role in apoptosis inhibition and Fe-S cluster biogenesis .
Biological Significance
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Fe-S Cluster Biogenesis: Fe-S clusters are essential cofactors for enzymes involved in DNA repair, electron transport, and redox regulation. Dysregulation of this process impacts plant growth and stress responses .
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Anti-Apoptotic Activity: The AT5G18400 protein may mitigate programmed cell death during abiotic stress, offering insights into plant resilience mechanisms .
Research Gaps and Future Directions
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Mechanistic Insights: Detailed studies on how AT5G18400 regulates apoptosis in plants are lacking.
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Stress Response Pathways: Its role in drought, salinity, or pathogen resistance remains underexplored.
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Interaction Networks: Proteomic studies could identify binding partners involved in Fe-S cluster assembly .
Technical Considerations
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research indicates that NBP35 physically interacts with DRE2. PMID: 27801963
- Findings suggest that AtDRE2 plays a role in DNA methylation control related to maternal activation in the EDL, an epigenetic function previously unknown for this protein in any organism. [AtDRE2] PMID: 25197096
