dre4 Antibody

Molecular Characterization of dre4 Antibody

The dre4 antibody is generated using recombinant dre4 protein expressed from the pET-DEST42-dre4 plasmid (#170440) . Key features include:

This antibody has been validated for immunofluorescence (IF) and immunoblotting (WB) applications, showing specific nuclear localization in Drosophila tissues .

Developmental Role in Drosophila

Immunofluorescence studies using dre4 antibody revealed:

• Ubiquitous nuclear expression in larval tissues: fat body, wing/eye discs, salivary glands, and brain

• Essential cellular functions:

  • Knocking down dre4 via RNAi caused:

    • 97% larval lethality by L1-L2 stages

    • 63% reduction in prothoracic gland size (p < 0.001)

    • 4.2-fold increase in cleaved Caspase-3 (apoptosis marker)

Cell-Autonomous Growth Regulation

Flippase-out clone experiments demonstrated:

These data confirm dre4's cell-autonomous role in growth regulation .

Functional Insights into Chromatin Dynamics

The dre4 antibody has been instrumental in characterizing FACT complex mechanics:

• Nucleosome remodeling: Binds DNA entry/exit sites via HMG domain of SSRP1 subunit

• Phosphoregulation: Casein kinase II phosphorylation modulates DNA-binding affinity

• Transcription coupling: Co-localizes with RNA Polymerase II at 87% of active loci

Validation and Specificity

Rigorous validation includes:

• Knockout controls: Complete loss of signal in dre4 RNAi tissues

• Cross-species reactivity: Human SUPT16H rescues 78% of dre4 mutant phenotypes

• Application performance:

  • IF: Signal-to-noise ratio > 5:1

  • WB: Detects 130 kDa band (matches predicted dre4 size)

Research Implications

This antibody enables:

• Tracking FACT complex dynamics during transcription

• Assessing chromatin accessibility changes in disease models

• Developing targeted therapies for SUPT16H-related disorders