At1g54070 Antibody

Target Protein Overview

AT1G54070 is a dormancy/auxin-associated protein linked to epigenetic regulation and developmental processes in Arabidopsis thaliana. It interacts with ARGONAUTE 1 (AGO1), a key component of the RNA-induced silencing complex (RISC), suggesting a role in post-transcriptional gene regulation .

Epigenetic Regulation

• AT1G54070 co-immunoprecipitates with AGO1, implicating it in gene silencing pathways mediated by small RNAs .

• Its association with dormancy suggests involvement in chromatin remodeling or histone modification, though precise mechanisms remain under investigation .

Auxin Signaling

• Auxin-related phenotypes in Arabidopsis mutants highlight AT1G54070's potential role in hormone-mediated growth regulation. Experimental knockdown models show altered root development and stress responses .

Validation and Specificity

• The antibody’s specificity is confirmed through immunoprecipitation assays and cross-reactivity tests with related proteins (e.g., AGO1) .

• Batch consistency is validated using mass spectrometry and peptide-blocking experiments .

Future Directions

• Functional Studies: Elucidate AT1G54070’s role in AGO1-mediated silencing using CRISPR-generated mutants.

• Structural Analysis: Cryo-EM or X-ray crystallography could resolve its interaction domains with AGO1 .