THY-2 Antibody

Biological Functions

Thy-1.2 is multifunctional in immune and neural systems:

• T Cell Activation: Acts as a costimulatory molecule, enhancing IL-2 production and proliferation when paired with TCR signaling .

• Stem Cell Regulation: Modulates hematopoietic stem cell differentiation and adhesion .

• Neuronal Signaling: Facilitates axon growth and synaptic plasticity .

• Apoptosis and Homeostasis: Contributes to T cell survival and peripheral pool maintenance .

Research Applications

Thy-1.2 antibodies are indispensable tools in immunology:

Common Clones and Uses:

Experimental Workflows:

• T Cell Depletion: Intraperitoneal injection of 30-H12 (0.2–0.5 mg) depletes >95% of Thy-1.2+ cells .

• Flow Cytometry: Staining at ≤0.06 µg/test (e.g., 30-H12-PE conjugate) .

Critical Research Findings

1. Thy-1.2 in T Cell Signaling:

   • Cross-linking Thy-1.2 induces IL-2 secretion and costimulates TCR-driven responses, mimicking partial TCR signaling .

   • Antibody-mediated ligation (e.g., 30-H12) triggers Ca²⁺ flux in thymocytes, influencing activation thresholds .

2. Immune Evasion Caveats:

   • Thy-1.2+ tumor grafts in Thy-1.1 congenic mice (e.g., B6.PL) induce cytotoxic anti-Thy-1.2 antibodies, confounding adoptive T cell transfer studies .

3. Therapeutic Potential:

   • Thy-1.2 antibodies show promise in targeting cancer-associated stroma while sparing normal tissue .