DTX17 Antibody

Antibody Characteristics and Applications

DDX17 antibodies are employed in immunohistochemistry (IHC), Western blotting (WB), immunofluorescence (IF), and immunoprecipitation (IP). Below is a comparative analysis of commercially available antibodies:

*Mouse reactivity is sequence-based .

Key Notes:

• Monoclonal vs. Polyclonal: Monoclonal antibodies (e.g., ab180190) offer higher specificity, while polyclonal (e.g., PA5-26330) may detect multiple epitopes .

• Optimized Conditions:

  • Antigen Retrieval: Tris-EDTA buffer (pH 9.0) for IHC .

  • Blocking Buffers: 5% NFDM/TBST for WB .

Role in Cancer Biology

DDX17 antibodies have elucidated its involvement in oncogenesis:

• Breast Cancer: IHC detects DDX17 in breast carcinoma tissues, suggesting a role in tumor progression .

• Prostate Cancer: While not directly studied with DDX17 antibodies, microtubule dynamics (a DDX17-associated process) are implicated in docetaxel resistance .

RNA Processing and Cellular Regulation

• Splicing Machinery: DDX17 interacts with RNA-binding proteins to regulate alternative splicing .

• Stem Cell Biology: IP and ChIP studies reveal DDX17’s interaction with pluripotency factors .

Diagnostic and Therapeutic Potential

• Biomarker Detection: Flow cytometry using ab180190 identifies DDX17 in intracellular compartments of leukemia cells (e.g., K562) .

• Epitope Mapping: Linear epitopes in DDX17 could guide therapeutic antibody development .

Challenges and Considerations

• Cross-Reactivity: Polyclonal antibodies may bind non-specific targets; validate with knockout controls .

• Disulfide Bond Stability: Manufacturing processes (e.g., cell lysis) can reduce interchain disulfides, affecting antibody integrity .