DUSP6 Antibody
Description
Introduction to DUSP6 Antibody
DUSP6 antibodies are immunological reagents specifically designed to detect dual specificity phosphatase 6, a key regulatory enzyme involved in cellular signaling mechanisms. These antibodies serve as crucial tools for investigating DUSP6 expression, localization, and function in both normal physiological conditions and various pathological states . The target protein, DUSP6, plays a significant role in regulating mitogen-activated protein kinase (MAPK) signaling pathways, particularly through its interaction with extracellular signal-regulated kinase (ERK) .
DUSP6 antibodies are available in several formats, including monoclonal and polyclonal variants, each with specific applications and unique binding characteristics. The growing interest in DUSP6's role in disease mechanisms has led to the development of diverse antibody products optimized for different experimental techniques and research applications .
Monoclonal versus Polyclonal DUSP6 Antibodies
Both monoclonal and polyclonal DUSP6 antibodies are commercially available, each offering distinct advantages for specific research applications:
Monoclonal DUSP6 Antibodies:
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Generated from a single B-cell clone, resulting in antibodies that recognize a single epitope
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Examples include Abcam's rabbit recombinant monoclonal antibody [EPR129Y] (ab76310)
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Offer high specificity and consistent lot-to-lot reproducibility
Polyclonal DUSP6 Antibodies:
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Produced by immunizing animals with DUSP6 protein or peptide fragments
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Examples include Antibodies-Online's ABIN6261384 and Proteintech's 26071-1-AP
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Recognize multiple epitopes on the DUSP6 protein
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Provide high sensitivity through binding to different regions of the target protein
Host Species and Immunogen Characteristics
The following table summarizes key properties of commercially available DUSP6 antibodies:
Binding Specificity and Target Regions
DUSP6 antibodies are designed to target specific regions of the DUSP6 protein:
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Internal region antibodies: ABIN6261384 from Antibodies-Online
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Center region antibodies: ABIN7301596 from Antibodies-Online
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C-terminal region antibodies: Available from various manufacturers
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Specific amino acid sequence targeting:
Applications of DUSP6 Antibodies
DUSP6 antibodies have been validated for numerous research applications, enabling the study of this phosphatase in various experimental contexts.
Western Blotting (WB)
Western blotting represents one of the most common applications for DUSP6 antibodies. Published literature and manufacturer data indicate:
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DUSP6 typically appears as a band at approximately 40-42 kDa
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Successfully detected in various human cell lines (HepG2, PC-12) and tissue samples (mouse heart tissue)
Immunohistochemistry (IHC)
DUSP6 antibodies are frequently employed in immunohistochemistry to visualize protein expression in tissue sections:
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Antigen retrieval protocols often recommend TE buffer pH 9.0 or citrate buffer pH 6.0
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Validated on various tissue types, including mouse pancreas tissue
Additional Applications
DUSP6 antibodies have been validated for several other techniques:
DUSP6 in Cancer Research
DUSP6 antibodies have been instrumental in elucidating this phosphatase's complex roles in cancer biology:
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Tumor-suppressive functions identified in pancreatic cancer, non-small cell lung cancer, esophageal squamous cell carcinoma, nasopharyngeal carcinoma, and ovarian cancer
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Pro-oncogenic activity documented in human glioblastoma, thyroid carcinoma, breast cancer, and acute myeloid carcinoma
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Both roles observed in malignant melanoma depending on histological subtype
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Loss or gain of function effects highlight DUSP6's significance in carcinogenesis
The dual nature of DUSP6 in cancer has stimulated research into its potential as both a prognostic biomarker and therapeutic target. DUSP6 antibodies have been essential tools for these investigations, enabling researchers to assess protein expression levels and correlate them with clinical outcomes .
DUSP6 in MAPK Signaling Pathways
Research utilizing DUSP6 antibodies has revealed critical insights into MAPK signaling regulation:
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DUSP6 specifically targets and inactivates ERK family kinases through dephosphorylation
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Functions as a negative feedback regulator in the MAPK pathway
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Plays a pivotal role in regulating cellular processes including proliferation, differentiation, migration, and survival
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Aberrant MAPK signaling involving DUSP6 dysregulation contributes to carcinogenesis
Potential Therapeutic Applications
The growing understanding of DUSP6 biology facilitated by antibody-based research has highlighted several potential therapeutic applications:
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Development of DUSP6-targeted therapies for cancer treatment
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Use of DUSP6 expression as a prognostic marker for cancer diagnosis and treatment outcome evaluation
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Potential role in pain management therapies, based on DUSP6's function in alleviating chronic postoperative pain
Selection Criteria for DUSP6 Antibodies
When selecting a DUSP6 antibody for research, consider the following factors:
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Application compatibility: Choose antibodies validated for your specific technique (WB, IHC, IF, etc.)
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Species reactivity: Ensure the antibody recognizes DUSP6 from your experimental model organism
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Clonality: Select monoclonal for specific epitope detection or polyclonal for enhanced sensitivity
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Validation data: Review published literature and manufacturer validation data
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Target region: Consider which domain of DUSP6 is relevant to your research question
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Format and conjugation: Select appropriate conjugation based on your detection method
Optimization Recommendations
For optimal results with DUSP6 antibodies:
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Follow manufacturer-recommended dilutions for each specific application
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Validate antibody specificity through appropriate controls, including knockout samples when available
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For immunohistochemistry, optimize antigen retrieval conditions (TE buffer pH 9.0 or citrate buffer pH 6.0)
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For western blotting, expect bands at approximately 40-42 kDa
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Titrate antibody concentrations when working with new experimental systems
Product Specs
Target Background
- Individuals carrying DUSP6 rs2279574 AA and AC genotypes are associated with an increased risk of developing lung squamous carcinoma in Han Chinese and with advanced NSCLC stages. PMID: 29578153
- DUSP6 expression in skeletal muscle is reduced by 43% immediately after exercise and remains below pre-exercise levels after a 2-hour recovery period. PMID: 28989118
- Data indicates that oxidative stress and MAP kinase phosphatase 3 (MKP3) inhibition play a pivotal role in procyanidin B2 3,3''-di-O-gallate (B2G2)-induced cell death in prostate cancer (PCa) cells through sustained activation of both ERK1/2 and AMPKalpha. PMID: 28876465
- High DUSP6 expression is associated with Lung Squamous Cell Carcinoma. PMID: 27613525
- Studies demonstrate the wide applicability of a recombination-based method and its potential to identify new drug targets through the identification of the tumor suppressor DUSP6 as a potential synthetic lethal target in melanoma cell lines with BRAF V600E mutations and high DUSP6 expression. PMID: 28423600
- PICSAR contributes to cSCC progression by activating the extracellular signal-regulated kinase 1/2 signaling pathway through downregulation of DUSP6 expression. PMID: 27049681
- DUSP6 rs2279574 SNPs were not associated with chemoradiotherapy response, whereas tumor regression, weight loss, clinical stage, and cigarette smoking were identified as independent prognostic predictors for Chinese patients with non-small cell lung cancer. PMID: 28231233
- DNA samples from each patient were genotyped for DUSP6 and TOP2A SNPs. PMID: 27156317
- Suppression of the FOXA1/DUSP6 signaling pathway may contribute to the development of Hirschsprung disease. PMID: 26097584
- Observations suggest that increased TSH signaling overcomes OIS and is crucial for B-RafV600E-induced papillary thyroid carcinogenesis. PMID: 25499223
- Dusp6 expression was elevated in progestin-sensitive atypical endometrial hyperplasia groups compared to progestin-resistant groups. After treatment, Dusp6 expression was upregulated in progestin-sensitive groups but not in progestin-resistant groups. PMID: 25451692
- Findings indicate that DUSP6 gene expression in tumor samples may serve as a prognostic marker in NSCLC. PMID: 25344212
- DUSP6 downregulated the expression of matrix metallopeptidase 3. PMID: 25241655
- Results indicate that p53-mediated up-regulation of MKP-3 contributes to the establishment of the senescent cellular phenotype by dephosphorylating ERK1/2. PMID: 25414256
- Downregulation of Dusp6, Sprouty4, and Sef--negative modulators of FGF2/ERK1/2 signaling--was observed in eutopic endometria of adenomyosis, which may play critical roles in the development of adenomyosis. PMID: 24681741
- Higher expression of DUSP6 in tumor tissue, compared to peritumor tissue, is associated with recurrence after curative resection of HCC, and the relative tumor DUSP6 expression has a strong ability to predict the recurrence of HCC. PMID: 24709168
- Findings suggest a crucial role for DeltaNp63alpha in preventing cancer metastasis by inhibiting Erk2 signaling through MKP3. PMID: 23246965
- Dusp6 overexpression is implicated in the pathogenesis and development of human endometrial adenocarcinoma. PMID: 23419500
- Increased DUSP6 expression is associated with papillary thyroid carcinoma. PMID: 24222120
- Depletion of DUSP6 reduced the viability of cancer cell lines and enhanced the cytotoxicity of EGFR and other targeted inhibitors, as well as cytotoxic agents. PMID: 23839489
- A missense mutation, c.545C>T (p.Ser182Phe), in the DUSP6 gene may be genetically linked to Class III malocclusion. PMID: 23965468
- DUSP6 is overexpressed in papillary and poorly differentiated thyroid carcinoma and contributes to neoplastic properties of thyroid cancer cells. PMID: 23132790
- Results provide insights into the modulatory role of p53 in the survival pathway by up-regulating DUSP6. PMID: 23108049
- A study reports that naive CD4 T cells from elderly individuals exhibit reduced signaling capacity of the ERK pathway; the underlying mechanism is an age-related decline in miR-181a expression and a corresponding increase in DUSP6 expression levels. PMID: 23023500
- Ischemia/reperfusion inhibited eNOS expression by inactivating ERK1/2, leading to decreased NO formation through a MKP-3-dependent mechanism in endothelial cells. PMID: 22848708
- DUSP6 plays a significant role in the pathogenesis of bipolar disorder, particularly in women. PMID: 22155192
- MESP2, HES7, and DUSP6 genes may not be involved in the etiopathogenesis of sporadic and non-syndromic CS in the Chinese Han population. PMID: 22744456
- A link between DUSP6 expression and high-risk features of papillary thyroid carcinoma suggests that DUSP6 is a crucial independent factor affecting the signaling pathways in established papillary thyroid carcinoma. PMID: 22535643
- DUSP6 is significant in melanoma and plays a distinct role in the different subtype of mouse melanoma compared to that in classic human melanoma. PMID: 22171919
- Caspase-3 cleavage of MKP3 down-regulates MKP3 full length and generates active MKP3 fragments, which may participate in novel regulatory pathways controlling the subcellular localization and activation of ERK1/2 during apoptosis. PMID: 22504224
- Studies identified a single gene, DUSP6, whose expression was associated with sensitivity to GSK1120212. PMID: 22169769
- Studies indicate that ERKs stimulate their own dephosphorylation by directly binding to phosphatases, such as MKP-3, and activating them. PMID: 22028468
- Loss of DUSP6 is associated with esophageal squamous cell carcinoma and nasopharyngeal carcinoma. PMID: 21387288
- DUSP6 plays a crucial role in cancer resistance in different subtypes of non-small cell lung carcinoma. PMID: 21680106
- Upregulation of DUSP6 exerts a tumor-promoting role in human glioblastomas, exacerbating the malignant phenotype. PMID: 21499306
- MKP3 not only controls the activities of ERK2 and p38alpha but also mediates cross-talk between these two MAPK pathways. PMID: 21454500
- This study reveals that post-transcriptional regulation is a key process in the control of DUSP6 expression. PMID: 20665674
- DUSP6 methylation is a rare occurrence in endometrial cancer. Silencing of the DUSP6 phosphatase is unlikely to contribute to constitutive activation of the ERK kinase cascade in endometrial cancer. PMID: 20638106
- Dual specificity phosphatase 6 (DUSP6) serves as an ETS-regulated negative feedback mediator of oncogenic ERK signaling in lung cancer cells. PMID: 20097731
- Angiotensin II-induced upregulation of MAP kinase phosphatase-3 mRNA levels mediates endothelial cell apoptosis. PMID: 11998972
- Results demonstrate that DUSP6 exerts apparent tumor-suppressive effects in vitro and suggest that DUSP6 is a strong candidate tumor suppressor gene at the 12q22 locus. PMID: 12759238
- Constitutive induction of p-Erk1/2 accompanied by reduced activities of protein phosphatases 1 and 2A and MKP3 due to reactive oxygen species during cellular senescence. PMID: 12840032
- The study provides the first direct evidence that the N-terminal domain of MKP3 mediates intramolecular dephosphorylation between the monomeric form of phosphorylated extracellular signal-regulated kinase and a monomer of MKP3. PMID: 14690430
- Cytoplasmic localization of MKP3 is mediated by a chromosome region maintenance-1 (CRM1)-dependent nuclear export pathway; the ability of MKP3 to cause the cytoplasmic retention of ERK2 requires both a functional kinase interaction motif and NES. PMID: 15269220
- Hypermethylation with modification of histone deacetylation plays a crucial role in transcriptional suppression of DUSP6 in human pancreatic cancer. PMID: 15824892
- Results suggest that the abrogation of DUSP6 is exclusively associated with progression from pancreatic intraepithelial neoplasia to invasive ductal carcinoma. PMID: 15832194
- Results demonstrate that in the Korean population, DUSP6 may contribute to the etiology of bipolar disorder, but not schizophrenia. PMID: 16491131
- Nuclear matrix proteins such as mutant Pyst1 and nucleophosmin 1 were downregulated, whereas eIF6 and beta-tubulin were upregulated during cell differentiation in hepatocarcinoma cells. PMID: 17569113
- Even upon overexpression, DUSP6 fails to inactivate ERK5, confirming that it is indeed an ERK1/2-specific DUSP. PMID: 18280112
- MKP3 can enhance DAT function and is a phosphatase involved in regulating dynamin-dependent endocytosis. PMID: 18434601
