DVL1P1 Antibody, Biotin conjugated

Applications in Research

This antibody is validated for ELISA and Western blotting (WB), with protocols optimized for high specificity .

Key Applications:

• ELISA: Used in sandwich assays to quantify native DVL1P1 in biological samples (e.g., serum, tissue homogenates) . The biotin-streptavidin system enhances sensitivity, with detection limits typically in the picogram range .

• Western Blotting: Detects DVL1P1 at dilutions of 1:500–1:2000, though validation data specific to DVL1P1 remains limited compared to its homolog DVL1 .

Research Findings and Utility

• Wnt Signaling Studies: DVL1P1 shares structural motifs with DVL1, a key mediator of Wnt/β-catenin and planar cell polarity pathways . While DVL1P1’s functional role remains unclear, its antibody enables pseudogene-specific investigations in cancer and developmental biology .

• Proteomic Profiling: Biotin conjugation allows integration with streptavidin-HRP or fluorescent tags, facilitating single-cell proteomics and multiplexed assays .

Limitations:

• Specificity Challenges: Cross-reactivity with DVL1 is a concern due to sequence homology . Negative controls (e.g., isotype-matched antibodies) are essential .

• Limited Validation: Published data on DVL1P1-specific applications are sparse compared to DVL1, necessitating user optimization .

Emerging Applications

Recent advances in spatial proteomics and cell-surface biotinylation leverage this antibody for neuron-astrocyte interaction studies and Wnt pathway dysregulation analyses . Its compatibility with DNA-barcoded streptavidin (e.g., TotalSeq) supports single-cell sequencing workflows .