E2FE Antibody
Description
E2F Family and Antibody Targets
The E2F family regulates cell cycle progression, DNA replication, and apoptosis. Antibodies against these proteins are critical for studying their roles in tumorigenesis and drug resistance.
E2F Antibodies in Cancer Research
E2F antibodies are pivotal in elucidating the family’s role in cancer progression and therapy resistance.
Mechanistic Insights
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Cell Cycle Regulation: E2F1/2/3 drive G1/S transition and inhibit apoptosis, promoting tumor growth .
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DNA Damage Response: E2F8 suppresses DNA repair pathways, enhancing genomic instability .
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Drug Resistance: Overexpression of E2F2/3/8 correlates with chemotherapy resistance, suggesting their role in multidrug resistance .
Antibody Validation and Techniques
Antibodies are rigorously tested for specificity and sensitivity:
Therapeutic Implications
E2F antibodies are tools for developing targeted therapies:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
This antibody targets E2FE, an inhibitor of E2F-dependent gene expression activation. E2FE specifically binds to the E2 recognition site without interacting with DP proteins, thereby preventing transcriptional activation by E2F/DP heterodimers. Its function includes controlling the timing of endocycle onset and inhibiting endoreduplication.
E2FE's role is supported by the following research findings:
- Del1 Knockout Mice and Osteoarthritis: Deletion of the Del1 gene (a homolog of E2FE) in mice resulted in more severe osteoarthritis, correlating with increased chondrocyte apoptosis. (PMID: 27505251)
- Nematode Feeding Site Generation: E2FE homologs are implicated in the development of nematode feeding sites. (PMID: 23518580)
- Arabidopsis Development: The down-regulation or overexpression of CCS52 and DEL1 (E2FE homolog) in Arabidopsis impacted giant cell and syncytium growth, hindering nematode development. This highlights the importance of mitotic activity and endoreduplication in feeding site maturation. (PMID: 22640471)
- Endoreduplication Regulation: E2FE overexpression does not completely abolish early endoreduplication under stress conditions. (PMID: 22535421)
- E2F Transcriptional Target: The Arabidopsis thaliana DEL1 gene has been identified as a transcriptional target of the E2Fb and E2Fc transcription factors. (PMID: 21908689)
- DNA Repair Gene Regulation: DEL1 acts as a transcriptional repressor of the PHR1 gene, involved in DNA repair. (PMID: 21131907)
- Endocycle Inhibition: DEL1/EL3, an E2FE homolog, is a significant inhibitor of the endocycle, maintaining the mitotic state of proliferating cells by suppressing genes required for entry into the endoreduplication cycle. (PMID: 15649366)
- CCS52A2 Gene Expression: The E2FE homolog E2Fe/DEL1 regulates the expression of the CCS52A2 gene in Arabidopsis thaliana. (PMID: 18787127)
