E2FF Antibody

E2F Antibodies: Overview and Family-Specific Targets

E2F transcription factors regulate cell cycle progression, DNA replication, and apoptosis. Antibodies targeting these proteins are critical for studying their roles in cancer, cell proliferation, and therapeutic responses. Key family members include:

Monoclonal vs. Polyclonal Antibodies

• Monoclonal Antibodies:

  • E2F-3 (Clone 3E2F04/PF37): Mouse IgG2a, detects nuclear/cytoplasmic E2F-3 in immunofluorescence and gel supershift assays .

  • E2F-4 (E3G2G): Rabbit mAb, validated for Western blot (WB), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP) .

• Polyclonal Antibodies:

  • Phospho-E2F-1 (Ser364): Detects phosphorylated E2F-1 in human samples, specific to the phosphorylated form .

  • E2F-2 (AF5119): Goat polyclonal, detects nuclear E2F-2 in Western blots (~70 kDa) .

Validated Applications

Role in Cancer Progression

• E2F-2: Overexpression correlates with invasive tumors and poor prognosis in lung adenocarcinoma; regulates cell growth, cycle progression, and motility .

• E2F-3: Linked to colon cancer aggressiveness; high expression predicts metastasis and survival outcomes .

• E2F-1: Phosphorylated E2F-1 (Ser364) is a marker of estrogen deprivation resistance in ER+ breast cancer; associated with CDK inhibitor sensitivity .

Therapeutic Implications

• Deregulated E2F Activity: Cancer cells exploit E2F signaling for proliferation but may be targeted via CDK inhibitors or therapies exploiting ARF-p53 pathway defects .

• E2F Score: A biomarker predicting chemotherapy response in ER+/HER2- breast cancer; correlates with CDK-related gene expression and immune checkpoint activity .