Customize E7 Antibody

Description

Definition and Biological Context

The E7 antibody refers to monoclonal or polyclonal antibodies specifically designed to detect and bind the HPV E7 oncoprotein. This viral protein induces malignant transformation by degrading tumor suppressor proteins like retinoblastoma (Rb), enabling uncontrolled cell division . Antibodies against E7 are essential for studying HPV-related cancers (e.g., cervical, head and neck) and developing diagnostic/therapeutic strategies.

Key steps in antibody development:

Antigen selection: Recombinant E7 proteins from high-risk HPV strains (e.g., HPV16, HPV18) are used as immunogens .
Screening: Antibody clones are selected via ELISA for specificity against target HPV strains while excluding cross-reactivity with low-risk HPV (e.g., HPV6, HPV11) .
Validation:
- Western blot: Detects endogenous E7 (~20 kDa) in HPV-positive cell lines (e.g., CaSki for HPV16, HeLa for HPV18) .
- Immunoprecipitation: Confirms binding to native E7 in cell lysates .
- Immunohistochemistry (IHC): Demonstrates E7 expression in clinical specimens, often localized to p16-positive areas .

Table 1: Common Applications of E7 Antibodies

Application	Description	Example Findings
Western Blot	Detects E7 in cell lysates	HPV16 E7 detected in CaSki cells; HPV18 E7 in HeLa cells .
IHC/ICC	Identifies E7 expression in FFPE tissues or liquid-based cytology (LBC)	Partial overlap with p16 staining in HPV16 DNA-positive tumors .
ELISA	Quantifies anti-E7 antibodies in serum	Rising anti-E7 levels correlate with cancer recurrence (100% specificity) .
Immunotherapy	Targets E7 in preclinical tumor models	Anti-E7 mAbs inhibit tumor growth and enhance complement deposition .

Prognostic Biomarker in HPV+ Cancers

Head and neck cancer (HNC): Patients with increasing anti-E7 antibody levels during treatment had higher mortality/recurrence rates (36.36% sensitivity, 100% specificity) .
Cervical cancer: E7 antibodies distinguish malignant transformations from transient infections, aiding early diagnosis .

Technical Challenges

Translational regulation: High E7 mRNA levels in HPV16+ specimens do not always correlate with protein expression, suggesting post-transcriptional control .
Cross-reactivity: Some antibodies (e.g., bs-10446R) detect multiple HPV strains (e.g., HPV16 and HPV35) .

Table 2: Comparison of Key E7 Antibodies

Product/Clone	Target	Host	Applications	Key Features	Source
ab100953 [8E2]	HPV18 E7	Mouse	WB, IP, IF, ELISA	Detects native E7 in HeLa cells .	Abcam
bs-10446R	HPV16/35 E7	Rabbit	IHC, WB, ELISA	Reacts with HPV16 and HPV35 E7 .	Bioss
NBP2-37628 [6F3]	HPV E7 (1-98)	Mouse	WB, IHC, ELISA	Binds recombinant E7 fragment .	Bio-Techne
DSHB E7	Beta-tubulin*	Mouse	IF, IP, WB	Cross-reactive with tubulin across species .	DSHB

Note: The DSHB E7 antibody targets beta-tubulin, not HPV E7, and is included here due to nomenclature overlap .

Future Directions

Biomarker panels: Combining E7 with p16 or circulating HPV DNA may improve diagnostic accuracy .
Therapeutic development: Anti-E7 antibodies conjugated to cytotoxic agents or used in combination with checkpoint inhibitors show preclinical promise .
Standardization: Efforts to harmonize assay protocols (e.g., ELISA thresholds) are needed for clinical adoption .

Product Specs

Buffer

**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (12-14 weeks)

Synonyms

E7Protein E7 antibody

Target Names

E7

Uniprot No.

P36831

Target Background

Function

The E7 protein plays a crucial role in viral genome replication by stimulating the entry of quiescent cells into the cell cycle. This stimulation of progression from the G1 to the S phase enables the virus to efficiently utilize the cellular DNA replicating machinery for viral genome replication. The E7 protein possesses both transforming and trans-activating properties. It induces the disassembly of the E2F1 transcription factor from RB1, leading to the subsequent transcriptional activation of E2F1-regulated S-phase genes. Additionally, E7 interferes with host histone deacetylation mediated by HDAC1 and HDAC2, resulting in transcription activation. Furthermore, E7 plays a role in inhibiting both the antiviral and antiproliferative functions of host interferon alpha. Its interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta).

Protein Families

Papillomaviridae E7 protein family

Subcellular Location

Host cytoplasm. Host nucleus.

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Source

Quantity&Size*

Product Name

Message

E7 Antibody