ECI2 Antibody, FITC conjugated
Description
Definition and Composition
ECI2 (Enoyl-CoA delta-isomerase 2) is a mitochondrial enzyme involved in the isomerization of enoyl-CoA intermediates during lipid metabolism. The FITC-conjugated ECI2 antibody is a rabbit-derived polyclonal IgG antibody that binds specifically to the recombinant human ECI2 protein (amino acids 119–219) . FITC (Fluorescein Isothiocyanate), a green-emitting fluorophore, is covalently attached to lysine residues on the antibody, enabling fluorescent detection.
Key Features:
Applications and Research Findings
The ECI2 Antibody, FITC conjugated, has been pivotal in studies linking ECI2 to disease mechanisms, particularly in cancer.
Role in Colorectal Cancer (CRC)
ECI2 exhibits tumor-suppressive properties in CRC by inhibiting:
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IL-8 Expression: Reduces interleukin-8 (IL-8) production, limiting neutrophil recruitment .
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Neutrophil Extracellular Traps (NETosis): Suppresses NET formation, a pro-tumorigenic process .
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Ether Lipid Synthesis: Inhibits alkylglycerone phosphate synthase (AGPS), reducing ether lipid intermediates .
Experimental Evidence:
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Immunofluorescence: Detects ECI2 in CRC cells and metastatic tissues, correlating with reduced neutrophil infiltration .
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Prognostic Value: Low ECI2 expression in CRC patients correlates with poor prognosis .
Mechanism of Action
ECI2 regulates lipid metabolism by isomerizing 3-cis and 3-trans enoyl-CoA to 2-trans enoyl-CoA, a step critical for β-oxidation and ether lipid synthesis . Its inhibition of AGPS activity reduces ether lipids, which are precursors for pro-inflammatory cytokines like IL-8 .
Table 2: Applications in Research
Considerations for Use
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Enoyl-CoA delta isomerase 2 (ECI2) has been identified as a novel androgen receptor (AR) target that promotes prostate cancer cell survival. PMID: 28415728
- Ectopic expression of the ACBD2/ECI2 isoform A in MA-10 cells resulted in increased basal and hormone-stimulated steroid formation. This suggests that ACBD2/ECI2-mediated peroxisomes-mitochondria interactions facilitate the exchange of metabolites and/or macromolecules between these two organelles, supporting steroid biosynthesis. Given the widespread occurrence of the ACBD2/ECI2 protein, it is proposed that this protein may play a role in the exchange of metabolites and/or macromolecules between peroxisomes and mitochondria. PMID: 27167610
- Disruption of mitochondrial beta-oxidation of unsaturated fatty acids has been observed in the 3,2-trans-enoyl-CoA isomerase-deficient mouse. PMID: 11916962
- DRS-1, a protein closely related to ECI2, has been identified as an autoantigen that may trigger immune attack against hematopoietic stem cells in a subset of acquired aplastic anemia patients exhibiting an increase in paroxysmal nocturnal hemoglobinuria-type cells. PMID: 15217832
