EEF1E1 Antibody
Description
Introduction to EEF1E1 Antibody
The EEF1E1 antibody (Catalog # NBP1-32184) is a polyclonal rabbit IgG antibody developed by Novus Biologicals, part of Bio-Techne, for research applications. It targets the EEF1E1 protein, a multifunctional molecule involved in translation elongation and DNA damage response pathways. The antibody is validated for use in immunocytochemistry (ICC), immunohistochemistry (IHC), immunohistochemistry-paraffin (IHC-P), and Western blotting (WB) across human and mouse samples .
Immunocytochemistry/Immunofluorescence
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Dilution: 1:100–1:1000
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Sample: HeLa cells (paraformaldehyde-fixed)
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Imaging: Cytoplasmic and nuclear localization observed, consistent with EEF1E1’s dual-compartment function .
Immunohistochemistry-Paraffin
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Dilution: 1:100–1:1000
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Sample: Mouse forebrain tissue
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Antigen Retrieval: Trilogy™ buffer (EDTA-based, pH 8.0) for 15 minutes .
Western Blotting
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Dilution: 1:500–1:3000
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Sample: A431 cell lysate (30 µg total protein)
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Observed Band: ~20 kDa (theoretical MW; actual may vary due to post-translational modifications) .
Research Findings and Clinical Relevance
The EEF1E1 protein has emerged as a critical biomarker in hepatocellular carcinoma (HCC). A 2021 study utilizing this antibody demonstrated:
Immune Microenvironment Impact
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Correlation with Immune Cell Infiltration: Positive associations were observed with Th2 cells (r = 0.306, p < 0.001) and NK CD56bright cells (r = 0.288, p < 0.001), while cytotoxic T cells (r = -0.247, p < 0.001) and dendritic cells (r = -0.250, p < 0.001) showed negative correlations .
p53 Signaling Pathway Involvement
Product Specs
Target Background
- LmnA binds AIMP3 via its extreme C-terminus. Together these findings provide a structural insight for understanding the interaction between AIMP3 and LmnA in AIMP3 degradation. PMID: 28797100
- AIMP3 has been shown to be involved in the cellular aging of human mesenchymal stem cells. PMID: 25465621
- EEF1E1 and OBFC2B have been identified as novel BRCA1-partner genes in the DNA double-strand break repair pathway. PMID: 24104880
- AIMP3 stabilizes and protects methionyl-tRNA synthetase and eIF2gamma through binding interactions. PMID: 23306449
- AIMP3 plays a critical role in mediating Met-tRNA(i)(Met) transfer from methionyl-tRNA synthetase to the eIF2 complex, ensuring accurate and efficient translation initiation. PMID: 22867704
- Research indicates that aminoacyl-tRNA synthetase-interacting multifunctional protein-3 (AIMP3)/p18 is released from aminoacyl-tRNA synthetase-interacting multifunctional protein-3 (AIMP3) due to UV irradiation-induced stress. PMID: 22106287
- Decreased expression of AIMP3 in gastric and colorectal cancer tissues suggests that down-regulation of this protein may be associated with inactivation of the tumor suppressor functions of AIMP proteins and potentially contribute to the development of gastric and colorectal cancer. PMID: 21789020
- p18 is a haploinsufficient tumor suppressor and a crucial factor for ATM/ATR-mediated p53 activation. PMID: 15680327
- Analysis of the three-dimensional structure and residues of the novel tumor suppressor AIMP3/p18 revealed the requirements for interaction with other proteins. PMID: 18343821
- No EEF1E1 mutations were detected in human cancer specimens. PMID: 19024604
