EIF2S2 (Ab-67) Antibody

Overview of EIF2S2 (Ab-67) Antibody

EIF2S2 (Ab-67) is a rabbit polyclonal antibody designed to detect endogenous EIF2S2 protein phosphorylation at Ser67. This post-translational modification plays a role in regulating EIF2S2’s function in protein synthesis initiation and cellular stress responses .

Protein Synthesis Studies

EIF2S2 is a subunit of the EIF2 complex, which forms ternary complexes with GTP and initiator tRNA to regulate translation initiation . The Ab-67 antibody enables the study of phosphorylation dynamics in this process, particularly under stress conditions (e.g., nutrient deprivation) .

Disease Research

While the antibody itself is not directly linked to clinical studies, EIF2S2 overexpression correlates with:

• Hepatocellular carcinoma (HCC) progression and poor prognosis .

• Pancreatic adenocarcinoma (PAAD) tumorigenesis via c-Myc pathway interactions .

Validation and Specificity

• Detects phosphorylation at Ser67 with high specificity .

• Validated in WB for human, mouse, and rat samples .

• Cross-reactivity predicted in pig, zebrafish, and bovine models .

Key Features

Phosphorylation and Signaling

Phosphorylation at Ser67 is mediated by casein kinase 2 (CSNK2A1) . This modification may influence EIF2S2’s interaction with other translation initiation factors, though mechanistic details remain under investigation .

Clinical Implications

• Immune infiltration correlation: EIF2S2 expression in HCC is linked to CD8+ T cells, PD-1, and CTLA-4 immune checkpoints .

• Drug sensitivity: High EIF2S2 levels correlate with increased sensitivity to paclitaxel and sunitinib in HCC models .

Limitations and Considerations

• Species restrictions: Limited reactivity outside mammals .

• Application scope: Primarily validated for WB; other applications (e.g., IHC, ELISA) require further testing .