ELM1 Antibody

Introduction to ELM1 Antibody

The ELM1 Antibody (clone ELM1) is a rat-derived IgG2a monoclonal antibody that specifically targets Nidogen-1, a sulfated glycoprotein critical for stabilizing basement membranes by bridging laminin and collagen IV networks . It is widely utilized in techniques such as immunoblotting, immunocytochemistry, and immunohistochemistry, with validated reactivity in murine species .

Key Applications and Protocols

The ELM1 Antibody has been optimized for diverse experimental workflows:

Sources:

Biological Significance of Nidogen-1

Nidogen-1 plays a structural role in basement membranes, which are essential for tissue organization and cell signaling. The ELM1 Antibody enables researchers to:

• Visualize basement membrane integrity in developmental and disease models .

• Study pathologies linked to basement membrane defects, such as kidney disorders and cancer metastasis .

• Validate in vitro extracellular matrix assembly assays .

Validation and Citations

• 15+ Citations: The ELM1 Antibody has been cited in peer-reviewed studies investigating extracellular matrix biology .

• Specificity: Confirmed via immunoblotting and immunofluorescence in murine tissues, with no cross-reactivity reported .

• Critical Controls: Includes validation against Nidogen-1 knockout models to confirm signal specificity .

Research Case Studies

While direct studies using the ELM1 Antibody are not detailed in the provided sources, its applications align with foundational work on basement membranes. For example:

• Cancer Research: Used to assess basement membrane disruption in tumor invasion assays .

• Developmental Biology: Employed in mapping basement membrane formation in murine embryos .

Limitations and Considerations

• Species Restriction: Reactivity is limited to mouse-derived samples .

• Optimal Dilution: Requires titration for each application to minimize background noise .