eltA Antibody
Description
ELTA Mechanism and Applications
ELTA leverages the enzyme OAS1 to catalyze the addition of a variety of chemical labels (e.g., biotin, fluorophores) to free or protein-bound ADP-ribose . This enzymatic approach replaces traditional chemical conjugation methods, which often yield low labeling efficiency (<20%). Key applications include:
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Fluorescence-based assays: Quantifying PAR (poly-ADP-ribose) polymer length and protein interactions.
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Mass spectrometry: Enriching ADP-ribosylated peptides for proteomic analysis.
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Imaging: Tracking PAR dynamics in live cells or tissues.
| ELTA Workflow | Key Steps |
|---|---|
| Sample preparation | Extract ADP-ribose or PAR-protein conjugates from cells/tissues. |
| Enzymatic labeling | Use OAS1 to add desired labels (e.g., biotin, Alexa Fluor 488). |
| Detection/enrichment | Use labeled probes for Western blot, fluorescence microscopy, or affinity purification. |
ELTA’s Relevance to Antibody Research
While ELTA itself is not an antibody, its utility intersects with antibody-based techniques in several ways:
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Antibody validation: ELTA can assist in verifying the specificity of anti-PAR antibodies by confirming the presence and localization of ADP-ribose modifications .
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Therapeutic antibody development: ADP-ribosylation is a key PTM in immune signaling and DNA repair. ELTA could aid in studying how therapeutic antibodies (e.g., PARP inhibitors) modulate these pathways .
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Biomarker discovery: ELTA enables precise detection of ADP-ribosylated proteins, which may serve as biomarkers for diseases like cancer or autoimmune disorders, complementing antibody-based diagnostics .
ELTA Sensitivity and Specificity
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ELTA demonstrates >90% labeling efficiency for free ADP-ribose and >70% for protein-conjugated PAR .
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Longitudinal stability studies show consistent labeling over 6 months when samples are stored at 4°C .
Applications in Disease Models
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Cancer research: ELTA has been used to study PARP1 activity in DNA repair pathways, critical for understanding tumor vulnerabilities to PARP inhibitors .
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Neurodegeneration: ELTA aids in mapping PAR signaling in neuroinflammatory contexts, such as Alzheimer’s disease .
Future Directions
ELTA’s versatility suggests potential for:
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
