ENPP5 Antibody

Structure and Function of ENPP5 Antibody

The ENPP5 antibody is typically produced in rabbit hosts as either polyclonal or recombinant monoclonal antibodies. Key structural features include:

• Reactivity: Cross-reacts with human, mouse, and rat samples .

• Applications: Validated for Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) .

• Epitope: Targets regions such as the C-terminal domain or unmodified protein sequences .

Table 1: ENPP5 Antibody Types and Suppliers

Cancer Expression

ENPP5 expression is analyzed in cancer through immunohistochemistry and Kaplan-Meier survival plots. High expression correlates with unfavorable prognosis in certain cancers (e.g., colorectal, breast) . The Human Protein Atlas reports protein expression in 20 cancer types, with representative images for tissues like colorectal and prostate cancer .

Functional Role

ENPP5 hydrolyzes NAD but lacks lysophospholipase D activity . Its role in neurotransmission is suggested by its ability to cleave NAD, a critical molecule in cellular energy metabolism .

Post-Translational Modifications

N-glycosylation has been identified as a key modification of the ENPP5 protein .

Western Blot

• Dilution: 1/1000–1/2000 for human cell lysates (e.g., MCF7, SH-SY5Y) .

• Predicted Band Size: 55 kDa .

Immunohistochemistry

• Tissue Staining: Used in paraffin-embedded sections of normal and cancer tissues (e.g., brain, breast) .

• Dilution: 1:50–1:200 for IHC .

Immunofluorescence

• Cell Lines: Tested in MCF7 cells (1/250 dilution) .