EPR1 Antibody
Description
Introduction to EPR1 Antibody
Effector cell protease receptor-1 (EPR1), also known as the receptor for the coagulation protease factor Xa, is a protein involved in regulating cellular processes and is found on the surface of various cells . EPR1 has a cysteine-rich extracellular module, a single membrane-spanning domain, and a serine-rich cytoplasmic tail with potential phosphorylation sites . It contains N-linked and O-linked glycosylation sites, as well as a chondroitin sulfate attachment site, which may anchor carbohydrate chains . EPR1 is expressed on activated platelets and megakaryocytes and is thought to mediate factor Xa binding to activated platelets, which forms a functional prothrombinase complex .
EPR1 as a Signal-Transducing Molecule
EPR-1 functions as a signal-transducing molecule in lymphocyte stimulation . Ligation of EPR-1 with factor Xa stimulates the proliferation of T and B lymphocyte subsets and enhances CD3-dependent lymphocyte proliferation . The activation of T cells that depends on EPR-1 results in early surface expression of the IL-2 receptor on target cells, increasing their responsiveness to low doses of IL-2 . Cross-linking of EPR-1 can also lead to a transient increase in cytosolic free calcium in T cells, which suggests its role in transmembrane signal transduction .
EPR1 and Cancer Research
EPR-1's role extends to cancer research, where it has been studied in the context of gastric adenocarcinoma . Research indicates that EPR-1 can influence the biological behaviors of tumor cells by inducing apoptosis and inhibiting proliferation .
EPR1 Antibody Specificity and Application
The EPR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 308-337 from the C-terminal region of human EPR1 . It demonstrates specificity for human EPR1 protein and is suitable for applications such as Western blot (WB) and ELISA (EIA) .
Comparative Analysis of EPR1 Antibody with Other Antibodies
| Antibody | Target | Host Species | Application |
|---|---|---|---|
| EP1 | Estrogen Receptor α (ERα) | Rabbit | Immunohistochemistry (IHC) |
| SP1 | Estrogen Receptor α (ERα) | Rabbit | Immunohistochemistry (IHC) |
| EPR1 | Effector Cell Protease Receptor-1 (EPR-1) | Rabbit | Western Blotting (WB), ELISA (EIA), Immunohistochemistry (IHC) |
Immunohistochemical Staining of Normal Tissues using Clone EP1
| SP1 | EP1 | # | Per cent | |
|---|---|---|---|---|
| Positive | Positive | 284 | 73.0 | |
| Positive | Negative | 4 | 1.0 | |
| Negative | Positive | 9 | 2.3 | |
| Negative | Negative | 93 | 23.9 | |
| Agreement | ||||
| 284/288 | =98.6% | |||
| 93/102 | =91.2% | |||
| 377/390 | =96.7% | |||
| κ (95% CI) | 0.912 | (0.866 to 0.959) |
