ERF15 Antibody
Description
Introduction to ERF15 Antibody
The "ERF15 Antibody" is not directly mentioned in the available literature, but it is likely related to antibodies targeting the ERF15 transcription factor. ERF15 is a transcription factor involved in plant responses, particularly in wound healing and regeneration processes in plants like Marchantia polymorpha . This article will focus on the ERF15 transcription factor and its relevance, as there is no specific information available on an "ERF15 Antibody."
ERF15 Transcription Factor
ERF15 is a wound-activated transcription factor crucial for gemmaling regeneration in Marchantia polymorpha. It plays a significant role in the wound response by controlling the biosynthesis of oxylipins such as OPDA (12-oxo-phytodienoic acid) and dn-OPDA (dihydrojasmonic acid), which are essential for regeneration .
Key Functions of ERF15:
-
Wound Response: ERF15 is instantly activated after wounding and is essential for tissue regeneration.
-
Oxylipin Biosynthesis: It regulates the expression of genes involved in oxylipin biosynthesis, such as MpAOS2 and MpAOC, leading to the accumulation of dn-OPDA.
-
Regeneration: The accumulation of OPDA and dn-OPDA is crucial for the regeneration process, and their absence leads to defective regeneration phenotypes.
Potential Use of Antibodies in ERF15 Research
Antibodies are crucial tools in molecular biology for detecting and studying proteins. While specific antibodies for ERF15 might not be widely documented, general principles of antibody use in plant biology could apply. Antibodies can be used for techniques like Western blotting, immunohistochemistry, and flow cytometry to study protein expression and localization.
Table: Potential Applications of Antibodies in Plant Biology
| Technique | Application | Description |
|---|---|---|
| Western Blotting | Protein Detection | Used to detect specific proteins in plant extracts. |
| Immunohistochemistry | Localization | Helps in visualizing the distribution of proteins within plant tissues. |
| Flow Cytometry | Cell Sorting | Can be used to sort cells based on specific protein markers. |
Research Findings and Implications
Research on ERF15 highlights its importance in plant wound response and regeneration. The hypersensitivity of MpERF15 mutants to OPDA and dn-OPDA suggests a complex regulatory mechanism involving these oxylipins . Understanding ERF15's role can provide insights into improving plant resilience and regeneration capabilities.
Table: Key Research Findings on ERF15
| Finding | Description |
|---|---|
| Wound Activation | ERF15 is instantly activated after wounding. |
| Oxylipin Biosynthesis | Controls the biosynthesis of OPDA and dn-OPDA. |
| Regeneration Role | Essential for gemmaling regeneration in Marchantia polymorpha. |
| Sensitivity to Oxylipins | MpERF15 mutants are hypersensitive to OPDA and dn-OPDA. |
Product Specs
Components: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Studies show that Arabidopsis thaliana ERF15 (At2g31230) overexpression lines with high expression levels exhibited slight growth retardation in early seedling development. PMID: 25253450
