AERO1 Antibody
Description
Introduction to AERO1 Antibody
The AERO1 Antibody is a mouse monoclonal antibody (MAb) targeting the ferric complex of aerobactin, a siderophore produced by certain bacterial pathogens like Escherichia coli. Isolated from E. coli strain KH576, it is classified as an immunoglobulin G (IgG) subtype 2 and exhibits high specificity for structurally related iron-chelating molecules . This antibody serves as a critical tool for detecting aerobactin and studying its role in bacterial pathogenesis, particularly in infections where iron acquisition is a virulence factor.
Key Properties
| Property | Details |
|---|---|
| Target | Ferric aerobactin (iron-chelating siderophore) |
| Epitope | Lysyl moiety of ferric aerobactin |
| Cross-Reactivity | Ferric arthrobactin, ferrioxamine B (no reactivity with other siderophores) |
| Detection Method | Competitive enzyme-linked immunosorbent assay (ELISA) |
| Sensitivity | Detection limit of 10 nM for purified ferric aerobactin |
The antibody’s specificity is attributed to its recognition of the lysyl group in ferric aerobactin, a structural feature shared with ferric arthrobactin and ferrioxamine B . This cross-reactivity highlights its utility in detecting diverse siderophores in clinical samples.
Efficacy Against Bacterial Pathogens
AERO1 Antibody demonstrates inhibitory effects on aerobactin-producing E. coli strains in newborn calf serum, a model mimicking iron-limited environments . By binding to ferric aerobactin, the antibody prevents iron delivery to bacteria, thereby starving them of essential nutrients and reducing growth.
| Model | Observation |
|---|---|
| Newborn Calf Serum | Reduced growth of aerobactin-producing E. coli |
| Clinical Isolates | Detects crude aerobactin in Enterobacteriaceae from bacteremia patients |
Diagnostic Applications
AERO1 enables quantitative detection of aerobactin via competitive ELISA, allowing researchers to monitor siderophore production in bacterial cultures or clinical specimens . This method is particularly valuable for studying aerobactin’s role in infections linked to iron metabolism, such as sepsis or urinary tract infections.
Potential Applications
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Anti-Infective Therapy: Targeting aerobactin could disrupt iron acquisition in pathogens, particularly in immunocompromised patients (e.g., cancer patients with bacteremia) .
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Diagnostic Biomarker: Rapid detection of aerobactin-producing strains may guide antibiotic selection.
Limitations
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In Vitro Data Only: Current efficacy data is limited to serum-based models; in vivo or clinical validation is pending.
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Cross-Reactivity: Reactivity with ferrioxamine B may complicate interpretation in environments where multiple siderophores are present.
