ESRRB Antibody, FITC conjugated
Description
Definition and Core Functionality
ESRRB Antibody, FITC Conjugated is a fluorescently labeled polyclonal antibody targeting Estrogen-Related Receptor Beta (ESRRB), a nuclear transcription factor critical for maintaining self-renewal in embryonic and trophoblast stem cells. The FITC (Fluorescein Isothiocyanate) conjugation enables fluorescence-based detection in applications such as immunofluorescence (IF), flow cytometry, and immunohistochemistry (IHC) . ESRRB regulates key genes like CDX2, EOMES, and ELF5, which are essential for stem cell pluripotency and differentiation .
Applications in Research
ESRRB FITC-conjugated antibodies are pivotal in:
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Stem Cell Studies: Tracking ESRRB’s nuclear localization in trophoblast stem cells (TSCs) and induced pluripotent stem cells (iPSCs) .
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Reprogramming Assays: Monitoring ESRRB overexpression during somatic cell reprogramming to TSCs .
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Flow Cytometry: Quantifying ESRRB-positive cells during early reprogramming stages .
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Immunofluorescence: Visualizing ESRRB in nuclear compartments (e.g., in piPSCs) .
Validation and Quality Control
The antibody undergoes rigorous validation:
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Specificity: Verified via Western blot (WB) in human heart tissue lysates, showing a single band at ~48–56 kDa .
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Cross-Reactivity: Confirmed in human, mouse, and rat samples using peptide-blocking assays .
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Functional Assays: Validated in ChIP-seq to identify ESRRB-bound genomic regions (e.g., CDX2 promoter) .
Table: Validation Metrics
| Assay | Result |
|---|---|
| Western Blot | Detected in PC-13 and A549 cell lysates |
| Immunofluorescence | Nuclear staining in human heart tissue and piPSCs |
| ELISA | Linear detection range established for quantitative analysis |
Research Findings
Key studies utilizing ESRRB antibodies include:
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Reprogramming Efficiency: ESRRB overexpression increases induced TSC colony formation by 8–10×, validated via flow cytometry (CD40+/Thy1– cells) .
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Transcriptional Regulation: ChIP-seq revealed ESRRB binds to enhancers of CDX2 and KRT8, activating trophoblast-specific genes .
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TGFβ Pathway Inhibition: ESRRB upregulates SMAD7 and MYC, promoting cervical cancer proliferation (validated via WB and IF) .
Comparative Analysis with Other ESRRB Antibodies
The FITC-conjugated variant offers unique advantages over other formats:
| Antibody Type | Applications | Advantages |
|---|---|---|
| FITC-Conjugated | IF, Flow Cytometry | Direct fluorescence detection; no secondary needed |
| Unconjugated | WB, IHC | Compatible with multiple secondary antibodies |
| HRP-Conjugated | ELISA, WB | High sensitivity for chemiluminescence |
Note: FITC-conjugated antibodies are optimal for live-cell imaging and high-throughput screening .
Limitations and Considerations
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Calcium levels were correlated with genetic variations in AMELX, AMNB, and ESRRB. AMELX and AMNB are involved in enamel mineralization. Mutations in both these genes are responsible for the amelogenesis imperfecta phenotype (OMIN), supporting their link to enamel alterations and mineralization. PMID: 28395292
- A SNP (rs61742642; C to T, P386S) within the ligand-binding domain of human estrogen-related receptor beta was associated with audiometric temporary threshold shift. PMID: 27399974
- ESRRbeta is mislocalized in human myocardium samples with idiopathic dilated cardiomyopathy, suggesting a potential role for ESRRbeta in the pathogenesis of this condition. PMID: 28130335
- The rs1676303 TT (P=0.02) and rs6574293 GG (P=0.04) genotypes of ESRRB were associated with RCD and TMD, respectively. PMID: 26584852
- Analysis of Esrrb target genes suggests Esrrb may be a significant factor in regulating cell proliferation. PMID: 26627478
- ERRbeta plays a role in estrogen-dependent cellular function, including cancer cell proliferation. PMID: 25805499
- The significant association and presence of high-risk haplotypes identified in the ESRRB gene confirm the association between variants in ESRRB and rotator cuff disease. PMID: 25219474
- Studies conclude that ESRRB, a gene associated with a form of hearing impairment, also contributes to dental decay, likely by influencing the formation of an enamel surface more susceptible to demineralization under acidic conditions. PMID: 25023176
- ERRbeta signaling leads to BCAS2-mediated blockage of the G1/S transition and inhibition of the epithelial to mesenchymal transition through FST-mediated regulation of E-cadherin. PMID: 24667650
- This is the first report of DFNB35 mutations in the Czech Republic, and it appears to be a rare cause of non-syndromic hearing loss. PMID: 22951369
- A novel missense mutation in the ESRRB gene causes DFNB35 hearing loss in a Tunisian family. PMID: 21802533
- Data confirms the up-regulation of ER-beta as the primary receptor involved in the progression of human endometriosis. PMID: 21561608
- ERRbeta plays a repressor role in the Nrf2-ARE pathway. PMID: 17920186
- ERRbeta performs a tumor suppressor function in prostate cancer cells. PMID: 18071305
- Data indicates that ESRRB is essential for inner-ear development and function, and a frameshift mutation in ESRRB results in non-syndromic hearing impairment. PMID: 18179891
- Sequence analysis of the ESRRB gene in affected individuals from the original DFNB35 family and three additional DFNB35-linked consanguineous families from Pakistan revealed four missense mutations. PMID: 18179891
- ERRbeta protein was localized to cell nuclei within multiple endometrial cell types, including the glands, stroma, endothelium, and immune cells, including uterine natural killer (uNK) cells and macrophages, throughout the normal menstrual cycle. PMID: 18775884
- Esrrb coordinates with Nanog and Oct4 to activate the internal machinery of ES cells. PMID: 18957414
- The short-form hERRbeta lacks an F domain and is the matched homolog of mouse and rat ERRbeta proteins in humans. However, hERRbeta2-Delta10 and the previously reported hERRbeta2 isoforms are primate-specific. PMID: 16332939
