EXOC8 Antibody, Biotin conjugated

Research Applications

This antibody is optimized for multiple experimental techniques, with validated performance in:

Western Blot (WB)

• Dilution Range: 1:1,000–1:6,000

• Detected Samples: Human hepatoma cells (HuH-7), mouse and rat testis tissues .

Immunofluorescence (IF)/Immunocytochemistry (ICC)

• Dilution Range: 1:50–1:500

• Validated in: Human epidermoid carcinoma cells (A431) .

ELISA

• Used for quantitative EXOC8 detection with streptavidin-HRP or streptavidin-AP systems .

Functional and Mechanistic Insights

EXOC8 is a critical subunit of the exocyst complex, which mediates vesicle docking and neurotransmitter release . Key research findings using this antibody include:

Interaction with Arl13b

• EXOC8 co-immunoprecipitates with Arl13b in a GTP-dependent manner, as demonstrated in ciliated IMCD3 and NIH-3T3 cells .

• The interaction is enhanced by GTPγS, indicating a role in GTPase-mediated exocyst regulation .

Role in Cellular Processes

• EXOC8 mutations disrupt exocyst assembly, leading to vesicle accumulation and neurodevelopmental disorders .

• Truncated EXOC8 causes defective cortical development, seizures, and brain atrophy .

Cross-Reactivity

Antibody Performance

• Signal Amplification: Biotin-streptavidin systems enhance detection sensitivity by ~10-fold compared to unconjugated antibodies .

• Specificity Control: No cross-reactivity observed with unrelated proteins in immunoprecipitation assays .

Recommended Workflow

1. Sample Preparation: Use RIPA buffer for protein extraction.

2. Antibody Incubation: Optimize dilutions using blocking buffers (e.g., 5% BSA).

3. Detection: Pair with streptavidin-HRP (for WB/ELISA) or streptavidin-fluorophores (for IF) .

Troubleshooting

• High Background: Reduce biotinylated antibody concentration or increase blocking time.

• Weak Signal: Titrate streptavidin-enzyme conjugates (typical range: 1:2,000–1:10,000) .