EXOC8 Antibody, HRP conjugated

Target and Biological Context

EXOC8 (Exocyst Complex Component 8) is an 84 kDa protein essential for tethering secretory vesicles to the plasma membrane during exocytosis. The exocyst complex, including EXOC8, regulates processes such as cell polarization, cytokinesis, and neurotransmitter release . Dysregulation of EXOC8 is implicated in cancer metastasis and neurological disorders .

HRP Conjugation Mechanism

HRP enables chemiluminescent or colorimetric detection by catalyzing substrate oxidation. Benefits of direct HRP conjugation include:

• Signal Amplification: Enhanced sensitivity compared to unconjugated antibodies .

• Workflow Simplification: Eliminates need for secondary antibodies in assays like ELISA .

• Compatibility: Works with substrates such as TMB (colorimetric) and luminol derivatives (chemiluminescent) .

ELISA

• Detects EXOC8 in serum or cell lysates with a working dilution determined by titration .

• HRP activity measured via absorbance at 450 nm using TMB substrate .

Western Blot

• Identifies EXOC8 at ~84 kDa in human cell lines .

• Requires optimization of blocking buffers to reduce background noise .

Comparative Analysis of EXOC8 Antibodies

The table below contrasts HRP-conjugated EXOC8 antibodies with other variants:

Validation and Quality Control

• Specificity: Validated using KO cell lines to confirm absence of non-specific bands .

• Batch Consistency: Protein A and peptide-affinity purification ensure lot-to-latility .

• Storage: Supplied in 50% glycerol/PBS with 0.03% ProClin 300, stable at -20°C .

Limitations

• Restricted to human samples; no cross-reactivity with mouse or rat .

• Not recommended for immunohistochemistry due to high background in fixed tissues .