EXPA10 Antibody

Introduction to EXPA10 Antibody

EXPA10 antibodies are monoclonal or polyclonal reagents that specifically target the EXPA10 protein, a member of the expansin family involved in pH-dependent cell wall loosening during plant growth . These antibodies are essential for elucidating EXPA10's localization, expression patterns, and functional roles in developmental and stress-response pathways .

Immunogen and Specificity

• Immunogen: Recombinant protein corresponding to Arabidopsis thaliana EXPA10 (UniProt: Q9LDR9) .

• Cross-reactivity: Confirmed in Arabidopsis thaliana, Cucumis sativus, Brassica rapa, Populus trichocarpa, and other plant species .

Biological Role of EXPA10

EXPA10 facilitates cell wall modification, enabling root elongation and syncytia formation during nematode infections . Key functions include:

• Cell wall loosening: Mediates pH-dependent wall extensibility in root cortex and elongation zones .

• Nematode interactions: Critical for syncytia development in Arabidopsis roots infected with cyst nematodes .

• Localization: Exhibits punctate distribution at three-cell boundaries in root apoplasts, particularly in cortex/epidermis junctions .

Hormonal Regulation

• Cytokinin and auxin: EXPA10 transcription shows limited responsiveness to exogenous cytokinins (e.g., 6-benzylaminopurine) or auxins (e.g., 1-naphthaleneacetic acid) .

• Expression patterns: Strongest in root cortex and lateral root transition zones, with negligible activity in meristematic regions .

Cell Wall Dynamics

• Localization: EXPA10 protein accumulates in longitudinal cell walls of elongated root cells, with distinct "spotty" patterns at intercellular junctions .

• Functional redundancy: Overlaps spatially with EXPA14 and EXPA15, suggesting cooperative roles in wall remodeling .

Applications in Research

• Mechanistic studies: Used to track EXPA10 dynamics during nematode infection or hormonal treatments .

• Cell wall imaging: Enables visualization of EXPA10’s subcellular localization via fluorescent tagging .

• Comparative analyses: Facilitates cross-species studies due to broad reactivity with orthologs in Brassica and Populus .

Future Directions

Current research gaps include EXPA10’s post-translational regulation and its interplay with other expansins. Advanced techniques like cryo-electron microscopy, coupled with EXPA10 antibodies, could resolve its structural interactions with cell wall components .