F13B Antibody, FITC conjugated
Description
Introduction to F13B Antibody, FITC Conjugated
F13B Antibody, FITC conjugated is a fluorescently labeled primary antibody designed for detecting coagulation factor XIII B subunit (F13B) in research applications. This antibody leverages fluorescein isothiocyanate (FITC) conjugation to enable visualization of F13B via fluorescence microscopy, flow cytometry, or immunoblotting. F13B, a non-catalytic subunit of factor XIII, stabilizes the active A subunits and regulates clot formation by crosslinking fibrin molecules .
Molecular Structure and Function of F13B
F13B is a 641-amino-acid glycoprotein containing 10 Sushi (CCP/SCR) domains, critical for stabilizing factor XIII tetramers in plasma . Key features include:
The antibody targets the internal region (AA 500-550) or middle region (AA 260-403) of F13B, depending on the product .
FITC Conjugation: Mechanism and Optimization
FITC conjugation involves covalent bonding of the isothiocyanate group to primary amines (e.g., lysine residues) on the antibody. Key parameters include:
Applications in Research
F13B Antibody, FITC conjugated is validated for:
Example Workflow:
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Sample Preparation: Lyse cells or plasma samples.
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Primary Antibody Incubation: Use FITC-conjugated F13B antibody at optimized dilution.
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Detection: Visualize fluorescence via microscopy or flow cytometry.
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Cross-reactivity: Ensure specificity for human F13B; some antibodies show partial reactivity with mouse, rat, or rabbit .
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Stability: Sodium azide must be removed before conjugation to prevent reaction interference .
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Background Noise: Higher FITC:antibody ratios (>6) increase nonspecific binding .
Research Findings and Implications
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Coagulation Studies: FITC-conjugated F13B antibodies enable real-time tracking of factor XIII activation in thrombin-treated plasma .
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Diagnosis of F13B Deficiency: ELISA-based detection aids in identifying patients with bleeding disorders linked to F13B mutations .
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Drug Delivery: FITC-labeled carriers (e.g., antibodies) monitor cellular uptake in targeted therapies .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Studies have revealed that in patients with venous thromboembolism (VTE), variations in FXIII levels and their impact on VTE risk exhibit significant sex-specific differences. The intron K polymorphism leads to reduced FXIII levels, but does not influence the risk of VTE. PMID: 28865246
- Research suggests that plasma FXIII levels are subject to multifaceted regulation, with age, fibrinogen level, and FXIII-B intron K polymorphism being primary determinants. These factors, through their influence on FXIII levels, may contribute to the risk of thrombotic diseases. PMID: 27821352
- Genetic markers associated with low FXIIIB levels are found to increase the risk of ischemic stroke of the cardioembolic subtype. PMID: 26159793
- The FXIII-B intron K nt29756 G allele has been associated with significant protection against coronary artery syndrome (CAS) and myocardial infarction (MI) in patients with a fibrinogen level in the upper tertile. PMID: 25569091
- Alterations in plasma levels of FXIIIB have been linked to cognitive decline in the elderly. PMID: 26088309
- This review provides an updated understanding of the pathophysiology of factor XIII deficiency and its therapeutic options. PMID: 24503678
- A case report describes congenital FXIII-B deficiency in which alloantibodies developed against exogenous FXIII-B. PMID: 23407795
- In eight Tunisian families with congenital factor XIII deficiency caused by two mutations, the FXIIIb subunit was found within normal range, while the expression of the FXIIIA subunit gene was decreased or undetectable. PMID: 19937244
- An ELISA/chemoluminescence assay has been developed demonstrating that FXIII-A and FXIII-B are low concentration components of the tear proteome. PMID: 20079358
- The role of FXIIIB in modifying the catalytic activity of FXIIIA2 during factor XIII-mediated crosslinking of fibrinogen has been investigated. PMID: 11816711
- Based on a study of family members of patients in South Asia, F13 B subunit antigen may play a role in susceptibility to stroke. PMID: 15634282
- Genetic variants of factor XIIIb have been evaluated for their effects on survival following myocardial infarction. PMID: 17515963
- It has been determined that at least three of the ten Sushi domains of FXIII-B possess the distinct function of forming a homodimer and a heterotetramer. This functionality is attributed to differences in their amino acid sequences. PMID: 18652485
- A specific colorimetric assay for measuring FXIIIB activity has been reported. PMID: 19646949
