FAAP20 Antibody
Description
Introduction
The FAAP20 antibody is a critical research tool for studying the Fanconi anemia-associated protein 20 (FAAP20), a key component of the Fanconi anemia (FA) pathway. This pathway is essential for repairing interstrand crosslinks (ICLs) and maintaining genomic stability. The antibody facilitates the detection and characterization of FAAP20 in cellular assays, immunoprecipitation, and chromatin localization studies.
Structure of FAAP20
FAAP20 is a 20-kDa protein containing a conserved ubiquitin-binding zinc-finger (UBZ) domain at its C-terminus, which binds K-63–linked ubiquitin chains in vitro . This domain is critical for its role in DNA damage response pathways, including recruitment to ICL sites and stabilization of the FA core complex .
Techniques Utilizing FAAP20 Antibody
The antibody is used in:
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Immunoprecipitation (IP): To isolate FAAP20 and associated proteins (e.g., FANCA, FANCG) from nuclear lysates .
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Immunoblotting (IB): To validate FAAP20 expression levels and interactions in wild-type vs. FA-deficient cells .
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Chromatin Immunoprecipitation (ChIP): To map FAAP20 recruitment to ICL sites, with enrichment observed at cross-linked DNA substrates .
Role of FAAP20 in DNA Repair Pathways
FAAP20 functions in:
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FA Core Complex Integrity: Stabilizes FANCA and facilitates chromatin loading of FANCA/FANCG subcomplexes .
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ICL Repair: Recruits to ICL sites via its UBZ domain, promoting FANCD2 monoubiquitination .
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Homologous Recombination (HR): Supports RAD51 focus formation and single-strand annealing (SSA), with FANCA-dependent and -independent roles .
Research Findings
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FANCA Dependency: FAAP20 stability is FANCA-dependent; knockdown of FANCA reduces FAAP20 levels via proteasomal degradation .
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Cancer Prognosis: Overexpression correlates with unfavorable outcomes in liver cancer and favorable outcomes in pancreatic cancer .
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Therapeutic Sensitivity: FAAP20-deficient cells show increased sensitivity to ionizing radiation and PARP inhibitors .
Interactions with Core Complex Proteins
FAAP20 interacts with:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research suggests that the Rev1 protein interacts with FAAP20 (Fanconi anemia-associated protein 20) in a specific manner. PMID: 26318859
- FAAP20 harbors a conserved ubiquitin-binding zinc-finger domain and exhibits the ability to bind K-63-linked ubiquitin chains in vitro. While this domain is not essential for interaction with FANCA, it plays a crucial role in the DNA-damage-induced chromatin loading of FANCA and the overall functional integrity of the Fanconi anemia pathway. PMID: 22343915
- Studies indicate that the interaction between FAAP20 and ubiquitin extends beyond the compact UBZ domain, requiring the folding and interaction of the otherwise disordered C-terminal tail of FAAP20 for high-affinity binding. PMID: 25414354
- Data suggest that both RNF8 and FAAP20 (C1orf86) are essential for efficient monoubiquitination of the Fanconi anemia group D2 protein, FANCD2. PMID: 22705371
- FAAP20 is a key player involved in the intricate Fanconi anemia pathway. PMID: 22396592
