FABP3 Antibody
Description
Introduction to FABP3 Antibody
Fatty Acid-Binding Protein 3 (FABP3), also known as heart-type FABP, is a 15 kDa cytoplasmic protein critical for transporting fatty acids and lipophilic substances (e.g., lysophosphatidic acid) to nuclear receptors like peroxisome proliferator-activated receptor gamma (PPARγ) . FABP3 antibodies are immunological tools designed to detect and quantify FABP3 in research and diagnostic applications. These antibodies are widely used in techniques such as Western blot (WB), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA) .
Cardiovascular Studies
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Ischemic Heart Injury: FABP3 antibodies revealed that FABP3 deficiency reduces cardiomyocyte apoptosis and improves cardiac remodeling post-myocardial infarction . Overexpression of FABP3 exacerbates heart dysfunction by activating pro-apoptotic MAPK pathways .
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Endothelial Function: FABP3 transports lysophosphatidic acid (LPA) to the nucleus in human coronary artery endothelial cells (HCAECs), facilitating PPARγ activation . siRNA-mediated FABP3 knockdown abolished this effect .
Cancer Research
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Non-Small Cell Lung Cancer (NSCLC): Elevated FABP3 expression correlates with advanced tumor stage and poor prognosis. Immunohistochemistry (IHC) using FABP3 antibodies demonstrated overexpression in 59.4% of NSCLC tissues compared to 9.9% in non-cancerous tissues .
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Dual Role in Tumors: FABP3 acts as a tumor suppressor in breast cancer but promotes aggressiveness in gastric carcinoma and NSCLC .
Neurological Disorders
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Synucleinopathies: FABP3 antibodies colocalized with α-synuclein (αSyn) aggregates in Parkinson’s disease (PD) and multiple system atrophy (MSA) brain tissues, suggesting a role in neurodegeneration . No association was observed with amyloid-β or tau pathology in Alzheimer’s disease .
Validation and Protocols
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Western Blot: FABP3 antibodies detect a single band at 15 kDa in heart tissue lysates .
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Immunohistochemistry: Used to localize FABP3 in cytoplasmic regions of cardiomyocytes and neurons .
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Functional Studies: siRNA knockdown experiments confirmed FABP3’s role in LPA transport and PPARγ activation .
Clinical and Therapeutic Implications
Product Specs
Fatty acid-binding protein heart, H-FABP, Heart-type fatty acid-binding protein, Muscle fatty acid-binding protein, M-FABP, Mammary-derived growth inhibitor, MDGI, FABP3, FABP11, O-FABP.
FABP3 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT9F10AT.
Anti-human FABP3 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FABP3 protein 1-133 amino acids purified from E. coli.
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
Fatty Acid Binding Proteins (FABPs) are a family of intracellular lipid chaperones that play a crucial role in the transport and metabolism of fatty acids. Among the nine identified members of the FABP family, Fatty Acid Binding Protein-3 (FABP3) is of particular interest due to its significant role in various physiological and pathological processes .
FABP3, also known as Heart-type FABP (H-FABP) or Muscle-type FABP (M-FABP), is predominantly expressed in cardiac and skeletal muscles. It is involved in the intracellular transport of long-chain fatty acids and other hydrophobic ligands, facilitating their delivery to sites of lipid metabolism . FABP3 has been implicated in several critical functions, including lipid signaling, storage, and oxidation .
FABP3 has garnered attention as a potential biomarker for various cardiovascular diseases. Elevated levels of FABP3 have been associated with myocardial infarction, ischemic heart injury, and other cardiac conditions . Research has shown that FABP3 contributes to cardiac myocyte apoptosis and remodeling after myocardial infarction, highlighting its role in the pathophysiology of heart diseases .
The development of mouse anti-human FABP3 antibodies has been instrumental in advancing research on FABP3. These antibodies are designed to specifically bind to human FABP3, allowing researchers to study its expression, function, and role in various diseases. The use of mouse anti-human FABP3 antibodies has facilitated numerous studies, providing insights into the molecular mechanisms underlying FABP3-related pathologies .
Mouse anti-human FABP3 antibodies have been widely used in various research applications, including:
- Western Blotting: To detect and quantify FABP3 expression in tissue samples.
- Immunohistochemistry: To visualize the localization of FABP3 in tissues.
- Enzyme-Linked Immunosorbent Assay (ELISA): To measure FABP3 levels in biological fluids.
- Flow Cytometry: To analyze FABP3 expression in cell populations.
These applications have significantly contributed to our understanding of FABP3’s role in health and disease .
