FAR3 Antibody

Biological Context of Far3 Protein

Far3 is part of a multi-protein complex in Saccharomyces cerevisiae localized to the endoplasmic reticulum (ER). Key roles include:

• Assembly Mechanism: Far3 interacts with Far9/10 (tail-anchored ER proteins) to recruit Far11, enabling tiered assembly of the Far complex .

• TORC2 Signaling: The complex regulates cell survival and lipid homeostasis via TORC2, with Far9’s tail-anchor domain critical for signaling efficiency .

Table 1: Components of the Yeast Far Complex

Antibody Structure and Function

While FAR3 antibodies are not explicitly detailed in the provided sources, general antibody principles apply:

• Structure: Composed of two heavy and two light chains forming Fab (antigen-binding) and Fc (effector function) regions .

• Mechanisms:

  • Opsonization: Fc regions bind macrophages/neutrophils to promote phagocytosis .

  • Complement Activation: IgG/IgM antibodies trigger membrane attack complexes .

Research Applications of FAR3 Antibodies

Hypothetical uses based on Far3’s role:

• Localization Studies: Detect Far3 in yeast ER membranes via immunofluorescence.

• Functional Knockdown: Block Far3 interactions to study TORC2 signaling defects.

Comparison to FGFR3 Antibodies

Though unrelated, FGFR3 antibodies highlight therapeutic antibody design principles:

• Epitope Specificity: FGFR3 antibodies target juxtamembrane (JMD) or tyrosine kinase (TKD) domains, correlating with neuropathy severity .

• Clinical Variability: Titers fluctuate without immunotherapy, complicating diagnostics .

Table 2: Antibody Classes and Functions

Antibody Databases and Resources

• IEDB: Hosts TCR/BCR sequences, including CDR3 regions .

• Antibody Database: Catalogs ~3.5 million sequences, including therapeutic antibodies .

Gaps and Future Directions

• No direct studies on FAR3 antibodies were identified in the reviewed literature.

• Leveraging structural databases (e.g., PDB) or NGS repositories could reveal unpublished sequences.