FAF4 Antibody

Mitochondrial Complex I Assembly Factor (NDUFAF4)

NDUFAF4 (NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 4) is a critical protein involved in the assembly of mitochondrial Complex I, part of the electron transport chain. While no direct reference to a "FAF4 antibody" exists in the search results, studies on its Drosophila homolog dNDUFAF4 (referred to as FAF4-kd in knockdown models) highlight its role in maintaining Complex I stability .

Key Findings:

• Structural Impact: Knockdown of dNDUFAF4 in Drosophila thoraces reduces Complex I activity by 35–40% and destabilizes subcomplexes (e.g., P P-a and P P-b modules) .

• Functional Redundancy: Both dNDUFAF3 and dNDUFAF4 knockdowns show overlapping but non-identical effects on mitochondrial function, suggesting partial redundancy .

Fibroblast Growth Factor 14 (FGF14/FHF4)

A commercially available monoclonal antibody (Clone N56/21) targets FGF14 (also termed FHF4), a neuronal protein regulating sodium channels. While distinct from "FAF4," this antibody is explicitly validated for research applications .

Anti-PF4 Antibodies in Thrombotic Pathologies

Though unrelated to "FAF4," anti-PF4 (platelet factor 4) antibodies are extensively studied in heparin-induced thrombocytopenia (HIT) and vaccine-induced thrombotic thrombocytopenia (VITT). These antibodies exhibit prolonged persistence (up to 15 months post-diagnosis) and are detected via ELISA or functional assays .

Critical Analysis:

• Ambiguity in Terminology: The term "FAF4" may stem from typographical errors (e.g., FGF14, FABP4, or NDUFAF4). None of the sources explicitly describe a "FAF4 antibody."

• Research Gaps: No studies in the provided materials focus on developing or characterizing an antibody specifically against a protein named "FAF4."

Recommendations for Further Investigation:

1. Verify the intended target (e.g., NDUFAF4, FGF14, or FABP4) to align with established antibody databases.

2. Explore proteomic repositories (e.g., UniProt, Antibodypedia) for commercial antibodies against NDUFAF4 or homologs.