FAM84B Antibody
Description
Introduction to FAM84B Antibody
FAM84B Antibody is a specialized immunological tool designed to detect FAM84B (Family With Sequence Similarity 84 Member B), a protein encoded by the FAM84B gene located on chromosome 8q24.21. This antibody is critical for identifying FAM84B expression in clinical and research settings, particularly in cancer studies where FAM84B amplification or overexpression is linked to aggressive tumor behavior and poor prognosis .
Applications in Cancer Research
FAM84B Antibody has been employed in diverse experimental and clinical contexts:
-
Immunohistochemistry (IHC): Used to detect FAM84B protein overexpression in esophageal squamous cell carcinoma (ESCC) and glioma tissues .
-
Western Blot: Validated FAM84B-NPM1 protein interactions in ESCC cell lines .
-
Flow Cytometry: Analyzed FAM84B’s role in cell cycle regulation (e.g., G1/S phase arrest) .
-
Co-Immunoprecipitation (Co-IP): Identified direct binding between FAM84B and nucleophosmin (NPM1) .
FAM84B in Glioma
-
Therapeutic Target: FAM84B siRNA reduced proliferation in U87 and T98 glioma cells by blocking the G0/G1 phase (P < 0.05) and downregulating Cyclin D1, CDK2, and CDK4 .
-
Clinical Relevance: Elevated FAM84B mRNA in glioma patients’ serum correlates with tumor progression .
FAM84B in Prostate Cancer
-
Metastasis Driver: FAM84B upregulation in castration-resistant prostate cancer (CRPC) is associated with reduced disease-free survival (DFS) and increased genomic copy number alterations .
Mechanisms of Action
FAM84B promotes oncogenesis through:
-
NPM1 Interaction: Enhances nuclear localization of NPM1, inhibiting CDKN2A (a tumor suppressor) .
-
Cell Cycle Regulation: Modulates Akt/GSK-3β/β-catenin and Wnt/β-catenin pathways in glioma and pancreatic cancer .
-
DNA Repair: Participates in DNA-repair complex formation, facilitating tumor survival .
Future Directions
FAM84B Antibody remains pivotal for:
