FAM92A Antibody
Description
Definition and Overview
FAM92A antibodies are polyclonal rabbit IgG antibodies developed against specific epitopes of the FAM92A1 protein (also known as CIBAR1). Two commercially available variants include:
These antibodies are validated for techniques such as Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF/ICC) .
Applications in Research
FAM92A antibodies have been instrumental in:
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Localization Studies: Identifying FAM92A at centrioles/basal bodies during ciliogenesis and synapses in neuronal tissues .
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Functional Knockdown: Demonstrating that FAM92A depletion impairs primary cilia formation in retinal pigment epithelial (RPE1) cells .
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Disease Modeling: Linking FAM92A loss-of-function variants to nonsyndromic postaxial polydactyly in humans and skeletal abnormalities in mice .
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Structural Analysis: Revealing FAM92A's BAR domain interactions with phosphoinositides and cardiolipin for membrane curvature induction .
Role in Ciliogenesis
FAM92A antibodies confirmed its interaction with Chibby1 (Cby1), a protein critical for basal body docking during cilia formation. Knockdown of FAM92A disrupts Rab8-positive membrane structures necessary for ciliogenesis .
Neuronal Development
In FAM92A1 knockout mice, these antibodies revealed:
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Reduced gray matter density in the entorhinal cortex and hippocampus .
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Abnormal mitochondrial cristae, synaptic ultrastructure, and myelin sheath integrity .
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Age-dependent cognitive deficits linked to impaired synaptic plasticity .
Genetic Disorders
A FAM92A frameshift mutation (c.490delC) was associated with polydactyly via disrupted ciliary localization of FAM92A and Cby1 .
Technical Considerations
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Specificity: The Proteintech antibody (24803-1-AP) loses reactivity in FAM92A1 knockout mice due to epitope deletion, whereas Sigma HPA034760 retains binding to residual exons 7–8 sequences .
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Validation: Both antibodies show orthogonal RNAseq validation but differ in non-specific band profiles during Western blotting .
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Experimental Design: Researchers must select antibodies based on target epitopes, especially when studying FAM92A1 knockout models .
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- FAM92 proteins interact with Cby1 to promote ciliogenesis by regulating membrane-remodeling processes. PMID: 27528616
- FAM92A1-289 exhibits various oncogenic properties, including facilitating cell migration, stimulating cell proliferation, and promoting colony formation in vitro and tumor growth in vivo. These findings suggest its potential as a therapeutic target for cancer treatment. PMID: 27798880
- FAM92A1 possesses different splicing variants and may play a regulatory role in cell proliferation and apoptosis. PMID: 17646714
- FAM92A1-289 is a newly identified tumor-related gene with oncogenic potential, possibly contributing to renal carcinogenesis. PMID: 19059705
