FARP1 Antibody
Description
Definition and Key Features of FARP1 Antibody
FARP1 antibodies are immunoreagents designed to detect FARP1, a protein containing:
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A FERM domain (linking cytoskeleton to membranes)
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A DH domain (Rho GTPase activation)
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PH domains (membrane targeting)
Common antibody characteristics ( ):
| Parameter | Details |
|---|---|
| Target Epitope | Varies (e.g., N-terminal AA 62–90, C-terminal AA 471–549) |
| Host Species | Rabbit, goat, or mouse |
| Clonality | Polyclonal or monoclonal (e.g., clone 2D4) |
| Applications | Western blot (WB), ELISA, immunofluorescence (IF), immunohistochemistry (IHC) |
| Conjugate Options | Unconjugated, HRP, FITC, or biotin |
Synaptic Development
FARP1 antibodies have been pivotal in uncovering its synaptogenic role:
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Postsynaptic Function: FARP1 binds SynCAM 1 via its FERM domain, promoting dendritic filopodia stability and spine formation in neurons .
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Rac1 Activation: Antibody-based assays show FARP1 activates Rac1 GTPase, driving F-actin polymerization in dendritic spines .
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Retrograde Signaling: FARP1 modulates presynaptic active zones by regulating SynCAM 1 clustering .
Cancer Biology
Studies using FARP1 antibodies reveal its oncogenic roles:
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Gastric Cancer: High FARP1 expression correlates with lymphatic invasion, metastasis, and poor prognosis (HR = 1.37, P = 0.025) .
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Mechanistic Insights:
Prognostic Marker
Immunohistochemistry (IHC) using FARP1 antibodies identifies it as a biomarker:
| Clinical Factor | Association with High FARP1 Expression |
|---|---|
| Lymphatic Metastasis | Significant (P = 0.012) |
| 5-Year Survival Rate | Reduced (P = 0.025) |
| Recurrence Rate | Elevated (P = 0.002) |
Functional Studies
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Knockdown/Overexpression: siRNA-mediated FARP1 silencing reduces cell motility by 31%, while overexpression increases filopodia formation via CDC42 .
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Actin Dynamics: FARP1 antibodies detect enhanced F-actin polymerization in filopodia and invadopodia .
Technical Considerations
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Specificity: Antibodies like clone 2D4 (AA 471–549) show no cross-reactivity with GST-tagged controls .
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Storage: Stable at -20°C for 6 months; sodium azide (0.09%) included as preservative .
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Validation: Peptide ELISA confirms detection limits up to 1:32,000 dilution .
Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
This CSB-PA897492LA01HU antibody is a polyclonal IgG generated by immunizing rabbits with a peptide corresponding to amino acids 1-300 of the recombinant human FARP1 protein. It is unconjugated and exhibits high reactivity with human FARP1 protein. FARP1 plays a crucial role in regulating neuronal development by interacting with cell-surface proteins such as class A Plexins and SynCAM 1. This antibody, purified using protein G, boasts a purity exceeding 95% and has been rigorously validated for use in ELISA, Western Blot (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF) applications.
Target Background
- Our research has demonstrated, for the first time, a reduced activity of both Rac1 and Cdc42 in human pheochromocytoma resection. Concurrently, we observed tumor-associated expression changes in FARP1, ARHGEF1, and ARHGAP36. PMID: 26911374
