At2g21930 Antibody

Biological Context of At2g21930

The At2g21930 gene is annotated as a pentatricopeptide repeat (PPR) protein, a family involved in RNA editing, splicing, and stability in plant organelles. PPR proteins are critical for chloroplast and mitochondrial function, impacting photosynthesis and stress responses. While the exact biological role of At2g21930 remains under investigation, orthologs of PPR proteins are linked to fertility and abiotic stress tolerance in plants .

Research Applications

The At2g21930 antibody has been utilized in:

• Epigenetic studies: Detection of histone modifications in endosperm nuclei during seed development .

• Transcriptional profiling: Validating protein expression in mutants with transposable element activation .

• Comparative analyses: Characterizing methylation patterns in Arabidopsis epigenetic mutants .

Validation Data

Key experimental findings:

• Western Blot: A single band at ~75 kDa (predicted molecular weight of At2g21930) .

• Immunoprecipitation: Successfully enriched At2g21930 in chromatin extracts during CUT&Tag experiments .

• Cross-reactivity: No observed binding to non-target Arabidopsis proteins in control assays .

Limitations and Future Directions

• Functional data: Limited studies directly linking At2g21930 to specific biochemical pathways.

• Structural insights: No crystallographic data available for the antibody-antigen complex.

• Comparative studies: Potential for expanded use in phylogenetically diverse plant species .