At1g20657 Antibody

Definition and Target

At1g20657 Antibody is a research-grade antibody targeting the protein encoded by the At1g20657 gene in Arabidopsis thaliana. The gene product, identified by the UniProt accession Q9LM89, is a putative F-box protein with a length of 422 amino acids . This antibody is primarily used in molecular biology to detect and study the protein’s expression, localization, and interactions in plant research.

Antibody Types

The antibody is available in two primary formats:

Key Features:

• Mouse Monoclonal Combinations:

  • X-Q9LM89-N: Targets N-terminal peptides (ELISA titer: 10,000; WB detection: ~1 ng) .

  • X-Q9LM89-C: Targets C-terminal peptides (identical performance to X-Q9LM89-N) .

  • X-Q9LM89-M: Targets non-terminal peptides (tested for WB) .

• Rabbit Polyclonal: Recognizes the full-length protein, enabling broader epitope coverage .

Primary Uses

1. Protein Detection:

   • Western Blot (WB): Validates protein expression levels in Arabidopsis tissues .

   • ELISA: Quantifies soluble At1g20657 in lysates or extracts .

2. Functional Studies:

   • Investigating F-box protein interactions in ubiquitination pathways (e.g., protein degradation, stress responses) .

   • Mapping subcellular localization (e.g., cytoplasmic/nuclear targeting) .

Example Workflow

Critical Considerations

• Validation Challenges: Commercial antibodies often lack rigorous validation (e.g., cross-reactivity with non-target proteins) . For At1g20657 Antibody, specificity should be confirmed via:

  • Negative Controls: Use Arabidopsis mutants lacking the At1g20657 gene .

  • Western Blot: Ensure no bands appear in knockout samples .

• Performance Variability: Mouse monoclonal combinations may offer higher precision, while rabbit polyclonal antibodies provide broader epitope coverage .

Supplier Comparison

Notes:

• Abmart: Offers pre-validated combinations under the AbInsure™ program (e.g., X2 -Q9LM89 for WB) .

• Cusabio: Provides rabbit polyclonal antibodies with glycerol-based storage (50% glycerol, PBS buffer) .