At3g13624 Antibody
Description
Introduction to At3g13624 Antibody
The At3g13624 Antibody (Catalog No. CSB-PA864761XA01DOA) is a polyclonal antibody developed for research applications involving Arabidopsis thaliana (mouse-ear cress), a model organism in plant biology . While specific studies involving this antibody are not detailed in publicly available literature, its design and production align with standard practices for plant protein-targeted antibodies.
Research Applications and Potential Uses
While direct evidence for At3g13624 Antibody’s utility is limited, antibodies targeting Arabidopsis genes typically serve roles such as:
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Protein Localization: Identifying subcellular distribution of At3g13624-encoded protein via immunofluorescence or immunohistochemistry .
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Gene Function Analysis: Studying gene knockouts or overexpression phenotypes in plant development or stress responses .
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Protein Interaction Mapping: Co-immunoprecipitation (Co-IP) to identify interacting partners in signaling pathways .
Antibodies in plant research often leverage techniques like Western blotting, which is implied by the antibody’s unconjugated form (commonly used in detection assays) .
Production and Purification Methods
Immunogen:
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Derived from a synthetic peptide corresponding to a region of the At3g13624 protein (exact epitope sequence unspecified) .
Purification Workflow:
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Antibody Production: Rabbit immunization with KLH-conjugated peptide (common for polyclonal antibodies) .
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Purification: Sequential protein A column chromatography and peptide affinity purification to ensure specificity .
This approach mirrors general antibody manufacturing processes, though specifics for At3g13624 are not detailed .
Comparative Analysis with Related Antibodies
The table below contrasts At3g13624 Antibody with other Arabidopsis-targeted antibodies from the same catalog :
| Antibody | Target Gene | Uniprot ID | Host | Application |
|---|---|---|---|---|
| At3g13624 Antibody | At3g13624 | Q9LHN6 | Rabbit | Western blotting |
| At5g38670 Antibody | At5g38670 | Q9FFV5 | Rabbit | Western blotting |
| At4g39580 Antibody | At4g39580 | Q9SVA0 | Rabbit | Western blotting |
Note: Applications are inferred from antibody descriptions; experimental validation is required.
Limitations and Future Directions
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Data Gaps:
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No peer-reviewed studies or experimental protocols involving At3g13624 Antibody are available in the provided sources.
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The gene’s biological role (e.g., metabolic pathways, stress responses) remains undefined in this context.
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Recommendations:
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Validate specificity via Western blotting or immunoprecipitation.
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Explore cross-reactivity with homologous proteins in other plant species.
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Product Specs
Components: 50% Glycerol, 0.01M PBS, pH 7.4
Q&A
Here’s a structured FAQ for researchers working with the At3g13624 Antibody in academic contexts, incorporating experimental design principles, methodological guidance, and advanced research considerations. Content is informed by antibody engineering best practices and technical validation approaches from peer-reviewed frameworks .
Advanced Research Questions
How can I engineer the At3g13624 Antibody for reduced immunogenicity in transgenic plant models?
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Species switching: Reformat the variable region into a plant-compatible backbone (e.g., Arabidopsis IgG-like scaffold) to minimize host immune recognition .
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Fc silencing: Introduce mutations (e.g., L234A/L235A) to disable effector functions while retaining binding .
What strategies improve At3g13624 Antibody utility in structural studies?
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Fragment engineering: Generate single-chain variable fragments (scFv) for cryo-EM compatibility (smaller size, improved penetration).
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Biotinylation: Site-specific conjugation via engineered cysteine residues enables streptavidin-based immobilization for SPR or AFM .
How do I resolve contradictions in At3g13624 subcellular localization data?
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Multi-method validation: Correlate IHC results with GFP-tagged At3g13624 lines and subcellular fractionation/Western blotting.
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Context-dependent analysis: Test antibody performance under stress conditions (e.g., drought, pathogen exposure) that may alter protein trafficking.
Methodological Considerations
7. Designing a At3g13624 knockdown-rescue experiment:
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Generate CRISPR/Cas9 knockout lines.
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Express At3g13624 cDNA under native/inducible promoters.
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Use engineered antibodies (e.g., Fc-silent variants) to track exogenous vs. endogenous protein without cross-detection .
8. High-throughput validation workflow:
| Step | Method | Purpose |
|---|---|---|
| 1 | Phage display library screening | Identify epitopes recognized by At3g13624 Antibody |
| 2 | Biolayer interferometry (BLI) | Quantify binding kinetics (KD, kon/koff) |
| 3 | Thermal shift assay | Assess antibody stability under varying pH/temperature |
Troubleshooting Data Conflicts
9. Discrepancies in At3g13624 protein abundance assays:
