At3g17620 Antibody
Description
Applications in Research
The At3g17620 antibody is validated for:
-
ELISA: Quantifying protein expression levels in Arabidopsis lysates.
-
Western Blot: Detecting the ~30 kDa protein (predicted molecular weight) in tissue extracts.
Research Limitations and Gaps
-
Functional Studies: No peer-reviewed studies directly utilizing this antibody in mechanistic or phenotypic experiments were identified in the provided sources.
-
Epitope Mapping: The exact epitopes recognized by this antibody are not disclosed, limiting precision in experimental design.
-
Comparative Data: Lack of cross-reactivity data with orthologs in other plant species (e.g., Oryza sativa or Zea mays).
Context in Antibody Development
The production of polyclonal antibodies involves immunizing host animals with recombinant proteins, followed by affinity purification . For At3g17620, the immunogen was synthesized recombinantly, ensuring specificity to the target protein. This contrasts with monoclonal antibodies, which require hybridoma technology for clonal selection .
Future Directions
Potential applications for this antibody include:
-
Subcellular Localization: Immunofluorescence microscopy in Arabidopsis tissues.
-
Protein-Protein Interaction Studies: Co-immunoprecipitation assays.
-
Phenotypic Analysis: Linking At3g17620 expression to stress responses or developmental traits.
Product Specs
Q&A
The At3g17620 antibody is a polyclonal reagent used in Arabidopsis thaliana research, primarily for detecting the At3g17620 protein via ELISA and Western blot . Below are research-focused FAQs addressing methodological and experimental design considerations.
Advanced Research Questions
How to resolve discrepancies in At3g17620 expression data across studies?
Can this antibody distinguish isoforms or post-translationally modified forms of At3g17620?
-
Perform 2D gel electrophoresis followed by WB to separate isoforms.
-
Use phospho-specific antibodies in parallel to identify modifications .
What strategies mitigate cross-reactivity in complex plant proteomes?
-
Immunodepletion: Preclear lysates with antibodies against high-abundance proteins .
-
Mass spectrometry validation: Confirm antigen identity after immunoprecipitation .
Technical Specifications Table
| Parameter | Detail | Source |
|---|---|---|
| Host species | Rabbit | |
| Reactivity | Arabidopsis thaliana | |
| Applications | ELISA, WB | |
| Storage | -20°C/-80°C; avoid freeze-thaw cycles | |
| Immunogen | Recombinant At3g17620 protein (UniProt Q9LUN5) |
Data Contradiction Analysis
-
Case example: If WB shows variable band sizes:
