At3g19560 Antibody
Description
Introduction to At3g19560 Antibody
The At3g19560 Antibody is a research reagent designed to target the protein encoded by the At3g19560 gene in Arabidopsis thaliana (mouse-ear cress). This antibody is commonly used in molecular biology to study gene expression, protein localization, and functional interactions in plant physiology, particularly in epigenetic regulation and stress responses.
Gene Expression and Epigenetic Studies
The At3g19560 Antibody has been utilized to investigate gene expression patterns in Arabidopsis, particularly in studies involving histone modifications and chromatin remodeling. For example:
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Gene Expression Analysis: Used to detect upregulation of At3g19560 in experiments involving histone demethylases. A study reported a 2.145-fold increase in gene expression under specific conditions, with antibody dilutions optimized at 1:170 for Western blotting .
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Functional Context: The target protein may participate in regulatory pathways linked to stress responses or developmental processes, though specific biochemical roles remain under investigation.
Experimental Design
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Antibody Validation: Typically validated for specificity in Western blot or immunoprecipitation assays.
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Cross-Reactivity: Limited to Arabidopsis thaliana based on current data.
Key Findings from Arabidopsis Studies
Product Specs
Q&A
Here’s a structured, research-focused FAQ collection for "At3g19560 Antibody" tailored to academic investigations, based on the provided scientific literature and methodological rigor:
Advanced Research Questions
Methodology:
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Spectral unmixing: Use confocal microscopy with narrow emission filters to separate signals from At3g19560 (e.g., Alexa Fluor 488) and secondary markers (e.g., lipid dyes like Nile Red).
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Sequential staining: Avoid simultaneous incubation with antibodies targeting epitopes in overlapping compartments (e.g., suberin vs. lignin).
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Data validation: Confirm results with RNAi lines or chemical inhibitors of lipid biosynthesis .
Analytical framework:
What integrative methods link AtHB25 function to seed longevity mechanisms?
Combine the At3g19560 antibody with:
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Lipidomics: GC-MS to quantify ω-hydroxy fatty acids and dicarboxylic acids in transgenic seeds .
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Transcriptomics: RNA-seq of AtHB25-OX lines to identify downstream targets (e.g., GPAT5, LACS2) .
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Epigenetics: ChIP-seq to map AtHB25 binding sites at promoters of lipid biosynthesis genes .
Table 1: AtHB25-associated pathways and experimental validation
Methodological Recommendations
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For low-abundance targets: Use chemiluminescent substrates with high dynamic range (e.g., SuperSignal West Femto) for Western blots.
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Quantitative imaging: Pair immunofluorescence with Fiji/ImageJ macros to normalize signal intensity against background autofluorescence.
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Data integration: Cross-reference antibody-based protein data with public RNA-seq datasets (e.g., Arabidopsis eFP Browser) to confirm transcriptional regulation.
