At3g21170 Antibody
Product Specs
Composition: 50% Glycerol, 0.01M PBS, pH 7.4
Q&A
FAQs for At3g21170 Antibody in Academic Research
Advanced Research Challenges
How to resolve contradictory data in At3g21170 protein localization studies?
Discrepancies often arise from:
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Fixation artifacts: Over-fixation masks epitopes; optimize permeabilization (e.g., 0.1% Triton X-100, 10 min) .
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Antibody cross-reactivity: Validate against Arabidopsis T-DNA insertion lines (e.g., SALK_012345) to confirm absence of off-target binding .
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Post-translational modifications: Use phosphatase/kinase inhibitors during extraction to preserve native protein states .
What computational tools enhance At3g21170 antibody experimental design?
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Epitope prediction: Use ABCpred or BepiPred to map antibody-binding regions on At3g21170 (Uniprot: Q9LM89) .
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Structural docking: Model antibody-antigen interactions with ClusPro for mutagenesis-guided validation .
Methodological Optimization
How to troubleshoot low signal-to-noise ratios in ChIP-seq using At3g21170 antibody?
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Chromatin shearing: Target DNA fragment size = 200–500 bp (confirmed by Bioanalyzer) .
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Blocking agents: 5% BSA + 0.1% fish gelatin reduces non-specific binding in plant tissues .
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Multiplex validation: Combine with RNA-seq to correlate protein occupancy with gene expression (e.g., stress-responsive genes WRKY53, HSFA2) .
Data Interpretation Frameworks
How to distinguish technical artifacts from biological variation in antibody-based assays?
Implement a decision tree:
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Reprodubility check: Repeat assay across 3 biological replicates.
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Orthogonal validation: Compare with CRISPR-Cas9 knockout lines or independent antibodies (e.g., anti-HA tagged At3g21170) .
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Quantitative thresholds: Define positivity criteria (e.g., ≥2-fold signal vs. IgG control) .
Emerging Applications
Can At3g21170 antibody inform evolutionary studies of stress adaptation?
Yes, through:
