At3g52320 Antibody
Description
Gene and Protein Structure
The AT3G52320 gene is located on chromosome 3 of Arabidopsis thaliana. Its open reading frame (ORF) spans 1,173 base pairs, encoding a 390-amino acid protein. Key features include:
| Property | Value |
|---|---|
| Gene ID | 824397 (NCBI Entrez) |
| Protein Name | F-box and associated interaction domains-containing protein |
| Protein Length | 390 amino acids |
| Molecular Weight | ~44 kDa |
| Domains | F-box domain (amino acids 50–90), Leucine-rich repeats (LRRs) |
Source: RefSeq (NM_115092.1, NP_190800.1) .
The F-box domain enables interaction with SKP1 proteins, forming part of the SCF (SKP1-CUL1-F-box) ubiquitin ligase complex. This complex tags proteins for degradation via the 26S proteasome, influencing processes like hormone signaling and stress responses .
Functional Studies
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Protein Localization: Antibodies against F-box proteins are used in immunofluorescence or immunohistochemistry to determine subcellular localization (e.g., nucleus, cytoplasm) .
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Expression Profiling: Western blotting or ELISA quantifies protein levels under stress conditions (e.g., drought, pathogen exposure) .
Interaction Networks
Co-immunoprecipitation (Co-IP) assays could identify binding partners, such as SKP1 or substrate proteins targeted for ubiquitination .
Comparative Analysis of Antibody Production Platforms
Hybridoma and recombinant antibody technologies are common for plant protein studies. Key platforms include:
Data synthesized from hybridoma and bispecific antibody studies .
Challenges and Opportunities
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Specificity: F-box proteins share structural homology, risking cross-reactivity. Epitope mapping (e.g., using peptide arrays) is critical .
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Therapeutic Potential: While Arabidopsis antibodies are primarily research tools, insights could inform crop engineering for stress resilience .
