At3g58860 Antibody

Definition and Functional Context

At3g58860 Antibody is a polyclonal or monoclonal antibody raised against the Arabidopsis thaliana protein encoded by the gene AT3G58860. This gene belongs to the F-box/RNI-like superfamily, which plays critical roles in ubiquitin-mediated protein degradation pathways . F-box proteins typically interact with E3 ubiquitin ligases to regulate protein turnover, influencing cellular processes such as stress responses, hormone signaling, and development .

The antibody is designed to specifically bind to the At3g58860 protein, enabling detection and analysis in experimental systems. Its primary applications include Western blotting, immunoprecipitation, and protein localization studies in plant biology research .

Gene Overview

Source: ThaleMine database

Protein Interaction Studies

At3g58860 Antibody is used to investigate interactions between F-box proteins and components of the ubiquitin-proteasome system. For example, it may aid in identifying substrates targeted for degradation by E3 ligases containing At3g58860 .

Hormone Signaling and Stress Responses

While direct evidence linking At3g58860 to abscisic acid (ABA) signaling is limited, F-box proteins are often involved in stress responses. A study screening for ABA-insensitive phenotypes in Arabidopsis utilized anti-FLAG antibodies (unrelated to At3g58860) to detect tagged proteins, highlighting a broader experimental context for F-box protein analysis in plant stress pathways .

Custom Antibody Development

Cusabio offers customized antibody services for At3g58860, including epitope selection and conjugation with tags (e.g., FLAG, HA) for enhanced detection .

Functional Insights

• Protein Degradation: As an F-box protein, At3g58860 may regulate turnover of transcription factors or enzymes involved in metabolic pathways.

• Stress Tolerance: F-box proteins are implicated in modulating plant responses to drought, salinity, and pathogens, though At3g58860’s specific role remains uncharacterized .

Technical Limitations

• Cross-reactivity: Limited data on antibody specificity (e.g., no reported immunogen sequence).

• Experimental Context: Most studies using At3g58860 Antibody focus on Arabidopsis, with no evidence of cross-reactivity in other species .