At4g17565 Antibody

Introduction to At4g17565 Antibody

The At4g17565 gene encodes a putative F-box protein, a component of E3 ubiquitin ligase complexes that regulate protein ubiquitination and subsequent degradation. The antibody is specifically raised against a recombinant At4g17565 protein and is used to study its expression and function in Arabidopsis thaliana .

Structure and Function of the Target Protein

F-box proteins are modular scaffolds that recruit substrate proteins to the SCF (SKP1-CUL1-F-box) complex, directing their ubiquitination. While the precise function of At4g17565 remains under investigation, F-box proteins in plants are often implicated in:

• Protein turnover during stress responses (e.g., temperature, drought).

• Developmental regulation (e.g., flowering time, cell differentiation).

• Pathogen defense, though specific roles vary by protein .

Applications and Experimental Uses

The antibody is primarily employed in Western Blot (WB) and ELISA assays to detect and quantify At4g17565 protein levels in Arabidopsis tissues. Potential research applications include:

• Studying protein degradation pathways in abiotic stress responses.

• Investigating developmental regulation (e.g., flowering time, senescence).

• Analyzing gene expression in transgenic or mutant Arabidopsis lines .

Cross-Reactivity and Specificity

The antibody is highly specific to At4g17565 in Arabidopsis thaliana, with no reported cross-reactivity to homologs in other species. This specificity is critical for minimizing false positives in downstream analyses .

Comparison with Other F-box Protein Antibodies

While F-box protein antibodies vary in target specificity and reactivity, the At4g17565 Antibody distinguishes itself through:

• Arabidopsis-specific reactivity, unlike antibodies targeting conserved F-box domains in animals or pathogens .

• Focus on plant biology, contrasting with antibodies for human F-box proteins (e.g., MAX.16H5 for CD4 ).

• Polyclonal design, offering broader epitope recognition compared to monoclonal alternatives .

Research Gaps and Future Directions

Despite its utility, gaps remain in understanding At4g17565’s functional role. Future studies could:

• Map epitopes to identify conserved regions for cross-species applications.

• Explore interactions with SCF complex components (e.g., SKP1, CUL1).

• Investigate stress-induced regulation via quantitative proteomics.