At5g03100 Antibody

Antibody Development and Applications

Though no specific data on the At5g03100 antibody exists in the provided sources, recombinant monoclonal antibody (mAb) workflows (e.g., hybridoma technology, phage display) are standardized for plant proteins . Key steps include:

1. Antigen Production: Expressing the At5g03100 protein or its epitope in bacterial/plant systems.

2. Immunization: Administering the antigen to host species (e.g., rabbits, mice).

3. Screening: Using ELISA or Western blot to identify high-affinity clones .

4. Validation: Testing specificity via knockout (KO) plant lines or immunoprecipitation .

Table 1: Common Antibody Validation Metrics (Based on General Standards )

Research Implications

Antibodies against plant F-box proteins like At5g03100 enable:

• Protein Localization Studies: Tracking subcellular dynamics via IF .

• Interaction Mapping: Co-immunoprecipitation (Co-IP) to identify binding partners (e.g., GI, ZTL) .

• Functional Knockdown: Antibody-mediated inhibition to study phenotypic effects (e.g., delayed flowering) .

Example Workflow from Homologous Systems :

1. Plant Material: Arabidopsis seedlings under controlled photoperiods.

2. Protein Extraction: Total protein isolation from shoots/roots.

3. Western Blot: Using At5g03100 antibody to detect expression levels.

4. Phenotypic Analysis: Correlating protein abundance with flowering time.

Challenges and Future Directions

• Characterization Gaps: ~50% of commercial antibodies lack sufficient validation .

• Cross-Species Reactivity: Antibodies for Arabidopsis proteins may not bind homologs in crops like Nicotiana .

• High-Throughput Demand: Projects like the EU’s Affinomics initiative aim to scale antibody generation for plant proteomes .