At5g25290 Antibody
Description
Overview of At5g25290 Antibody
The At5g25290 Antibody is a polyclonal antibody raised in rabbits against the At5g25290 protein from Arabidopsis thaliana (mouse-ear cress). This antibody is designed for research applications, particularly in studying gene expression and protein interactions in plant biology. Below is a detailed analysis of its characteristics, research applications, and experimental findings.
Gene Expression in Plant-Pathogen Interactions
The At5g25290 gene has been identified as a highly upregulated transcript during infection studies in Arabidopsis. In microarray analyses of plants infected with pathogens like Erysiphe cichoracearum (powdery mildew), At5g25290 emerged as the most upregulated gene, though its functional role remains uncharacterized . This suggests potential involvement in immune signaling or stress responses, though further studies are needed to confirm its biological significance.
Antibody Utility in Protein Detection
The antibody is validated for use in:
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ELISA: Quantitative detection of At5g25290 protein in plant extracts.
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Western Blot: Qualitative assessment of protein expression levels.
No cross-reactivity with non-Arabidopsis species (e.g., human, pig, bovine) has been reported, ensuring specificity .
Challenges and Future Directions
Despite its utility, At5g25290 Antibody faces limitations:
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Limited Functional Data: The At5g25290 protein lacks annotated biological functions, hindering interpretation of experimental results.
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Niche Applications: Primarily used in plant biology research, with no reported use in clinical or agricultural diagnostics.
Future research could leverage this antibody to:
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Investigate protein localization during pathogen attack.
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Screen for At5g25290 interactions with other proteins (e.g., transcription factors, enzymes).
