Customize At5g42430 Antibody

Description

Gene and Protein Characteristics

The At5g42430 gene encodes an F-box and associated interaction domains-containing protein involved in protein degradation via the ubiquitin-proteasome system . Key features include:

Attribute	Details
Gene ID	AT5G42430
UniProt ID	Q9FIH4
Protein Class	F-box protein; part of the SCF (Skp1-Cullin-F-box) E3 ubiquitin ligase complex
Molecular Function	Substrate recognition in ubiquitination pathways
Biological Process	Regulation of cellular processes (e.g., signal transduction, stress response)

F-box proteins typically mediate protein-protein interactions, targeting specific substrates for proteasomal degradation .

Research Applications and Targets

The At5g42430 antibody facilitates studies on:

Protein Localization: Subcellular distribution of the F-box protein in plant tissues.
Interaction Networks: Identification of binding partners in ubiquitination pathways.
Functional Knockdown: Assessing phenotypic changes in At5g42430 knockout mutants.

Identified Targets:
From chromatin immunoprecipitation (ChIP) and interaction studies, At5g42430 potentially regulates genes encoding :

Target Locus	Gene Product	Function
AT2G16750	Kinase with adenine nucleotide hydrolase-like domain	Metabolic regulation
AT5G05720	RNA-binding family protein	Post-transcriptional modification
AT1G44760	Adenine nucleotide alpha hydrolases-like protein	Nucleotide metabolism

Challenges in Antibody Specificity

While no direct data on At5g42430 antibody cross-reactivity exists, broader antibody validation challenges include:

Cross-Reactivity: Antibodies may bind unintended proteins of similar molecular weight (e.g., anti-GR antibody clone 5E4 binds AMPD2/TRIM28) .
Validation Methods: Recommendations include knockdown assays, immunocapture-mass spectrometry, and orthogonal validation .

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Composition: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

At5g42430 antibody; MDH9.12Putative F-box protein At5g42430 antibody

Target Names

At5g42430

Uniprot No.

Q9FIH4

Q&A

FAQs for Researchers on At5g42430 Antibody in Arabidopsis thaliana

Advanced Research Questions

How to resolve contradictions in At5g42430 antibody binding across different plant stress conditions?
- Analysis framework:
  - Conduct competitive ELISA with stress-induced protein extracts to test cross-reactivity with homologous F-box proteins .
  - Use cross-linking immunoprecipitation (CLIP) to identify interacting partners under specific stressors (e.g., drought, pathogen exposure) .
  - Compare RNA-seq data to confirm transcript levels align with protein detection trends .
What computational tools predict At5g42430 antibody-epitope binding dynamics?
- Approach:
  - Apply biophysics-informed models (e.g., RosettaAntibody) to simulate interactions between At5g42430’s F-box domain and antibody paratopes .
  - Validate predictions via alanine scanning mutagenesis of predicted epitope residues.

Data Integration & Validation

How to harmonize conflicting omics datasets involving At5g42430?
- Strategy:
  - Multi-omics correlation: Align proteomics (antibody-based) with transcriptomics (RNA-seq) and phosphoproteomics data to identify post-translational regulation .
  - Meta-analysis: Compare public datasets (e.g., TAIR, Araport) for consensus expression patterns across tissues/stages .

Technical Challenges

Why does At5g42430 antibody yield nonspecific signals in root tissue?
- Troubleshooting:
  - Test tissue-specific blocking buffers (e.g., 5% BSA + 2% plant-specific protease inhibitors).
  - Use fluorescence-activated cell sorting (FACS) to isolate root cell populations before immunodetection .

Functional Studies

How to dissect At5g42430’s role in ubiquitination pathways?
- Experimental pipeline:
  1. Perform co-IP/MS with At5g42430 antibody to identify E3 ligase complexes.
  2. Use in vitro ubiquitination assays with recombinant proteins (e.g., UBA1, UBC10) .
  3. Profile ubiquitination sites via LC-MS/MS in At5g42430 mutants.

Cross-Species Applications

Can At5g42430 antibody cross-react with orthologs in Brassica species?
- Validation steps:
  - Align At5g42430’s epitope region with Brassica proteomes (e.g., BLASTp).
  - Test antibody on heterologous expression systems (e.g., Nicotiana benthamiana transient assays) .

Table: Key Features of At5g42430

Property	Detail	Source
Gene ID	AT5G42430 (Arabidopsis thaliana)
Protein class	F-box and associated interaction domains
Subcellular localization	Nucleus/Cytoplasm (predicted)
Common interactors	SKP1-like proteins, Cullin scaffolds

Methodological Recommendations

For low-abundance targets, combine antibody-based enrichment (e.g., immunoprecipitation) with tandem mass spectrometry .
Address batch variability by including internal controls (e.g., constitutively expressed reference proteins) in all experiments .

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Source

Quantity&Size*

Product Name

Message

At5g42430 Antibody